不同来源乳酸菌的耐酸耐胆盐试验  

Tolerance Assays to Acid and Bile Salt of Lactobacillus from Different Sources

作　　者：杨晓宇[1] 张七斤[1] 于晨龙[1] 陈光明[1] 苏日娜[1] 吕天星[1] 

机构地区：[1]内蒙古农业大学兽医学院农业部动物临床诊疗技术重点实验室,内蒙古呼和浩特010018

出　　处：《动物医学进展》2014年第2期73-77,共5页Progress In Veterinary Medicine

基　　金：内蒙古自然科学基金项目(31160508)

摘　　要：试验从健康猪(非哺乳期仔猪)、鸡及传统乳制品中分离到30株乳酸菌,镜检、生化鉴定后对其中的6株高性能菌株进行耐酸耐胆盐试验。结果显示,所分得的30株乳酸菌中18株为乳杆菌,其余12株为乳球菌;LS和LJ2菌株在pH 2.0环境下的存活率分别为135%和124%;LZ1株在pH 3.0环境下的存活率为137%;LJ1株对猪胆盐有较高耐受能力,LT株对牛胆盐有较高耐受能力。这些高生存性菌株的获得有助于进一步研究乳酸菌的益生特性,也可以作为功能性菌株深入研究。30 strains of Lactobacillus were isolated from intestines of pigs and chickens, and traditional fermented milk products. After microscopy and biochemistry identification, 6 strains were used for acid and bile salt tolerance tests. The results showed that among 30 strains, 18 strains were Lactobacillus, others were Lactococcus. LS and LJ2 have a high survival rate for 135%and 124 under pH2. 0 growth environment, LZ1 can tolerate pH3.0 growth environment, its survival rate is 137 ; LJ1 has a higher tolerance to bile salt, LT has a higher tolerance to bovine bile salt. These high survivability strains can help to further study on the probiotic properties and functions of Lactobacillus .

关 键 词：乳酸菌 高生存性 耐酸 耐胆盐 

分 类 号：Q939.117[生物学—微生物学]