KRAS基因突变直接测序检测方法的建立  

Establishment of a sequencing method for detecting KRAS gene mutations

作　　者：容毓[1] 罗凯[1] 贺智敏[1] 

机构地区：[1]广州医科大学附属肿瘤医院广州医科大学肿瘤研究所,510095

出　　处：《国际医药卫生导报》2014年第2期172-175,共4页International Medicine and Health Guidance News

摘　　要：目的 建立一种适用于临床的KRAS基因直接测序突变检测方法.方法 以KRAS基因12、13密码子为突变检测靶位点设计特异性扩增、测序引物,以已知12、13密码子野生型、突变型样品分别做为阴、阳性对照品,建立KRAS基因突变直接测序检测方法,并行方法学评估.结果 成功建立了KRAS基因突变直接测序检测方法,该方法检测灵敏度达3.9 ng/μl,重复性良好.检测17例结直肠癌样品,突变率为23.5％.结论 本研究成功建立了可用于临床样品检测的KRAS基因突变直接测序检测方法.Objective To establish a sequencing method for detecting KRAS gene mutations. Methods A sequencing method for KRAS gene mutations detection was established with specific primers that targeted hotspot mutation region （codonl2 and codonl3）, and wild-type and mutation samples were selected as negative sample and positive sample respectively. Then the performance of the method was assessed. Results A sequencing method for KRAS gene mutations detection was established successfully which owned high sensitivity （3.9 ng/μl） and good repeatability. The mutation rate was 23.5% in 17 colorectal cancer samples. Conclusion The sequencing method can be used in clinical samples to detect KRAS genemutations.

关 键 词：KRAS 突变 基因测序 结直肠癌 

分 类 号：R440[医药卫生—诊断学]