Glypican3、MMP-9和MMP-14在原发性肝癌中的表达与临床意义  

Expressions and clinical significance of Glypican3,MMP-9 and MMP-14 in primary hepatocellular carcinoma

作　　者：刘敏[1] 曾霞[2] 侯恩存[1] 王树声[3] 

机构地区：[1]广西中医药大学附属瑞康医院肿瘤二科,南宁530011 [2]广西医科大学免疫学教研室,南宁530021 [3]广西疾病预防控制中心,南宁530028

出　　处：《重庆医学》2014年第2期173-176,共4页Chongqing medicine

摘　　要：目的探讨原发性肝癌中磷脂酰肌醇蛋白聚糖(Glypican3)、基质金属蛋白酶-9(MMP-9)和基质金属蛋白酶-14(MMP-14)的表达特征,关注其在肿瘤发生、发展和转移中的作用。方法本试验以102例原发性肝癌作为观察组,以80例正常肝组织作为对照组,采用免疫组织化学方法检测两组中Glypican3、MMP-9和MMP-14的表达,探讨Glypican3、MMP-9和MMP-14在不同临床病理特征中的表达差别及相关性。结果观察组中Glypican3、MMP-9和MMP-14表达的阳性率明显高于对照组,观察组中Glypican3、MMP-9和MMP-14表达的阳性率与肿瘤体积、分化程度、淋巴结转移、脉管浸润、被膜侵犯和增殖组胞核抗原的表达密切相关。相关性分析显示观察组中Glypican3和MMP-9、Glypican3和MMP-14、MMP-9和MMP-14的表达均呈正相关性(r=0.48,P=0.024 1;r=0.46,P=0.013 2;r=0.43.P=0.031 3)。生存分析显示Glypican3、MMP-9和MMP-14的表达与预后相关。结论原发性肝癌组织中Glypican3、MMP-9和MMP-14高表达,在肿瘤进展中有明显的促进作用,术后检测Glypican3、MMP-9和MMP-14的表达对判断原发性肝癌的预后有一定价值。Objective To investigate the expression characteristics of Glypican3,MMP-9 and MMP 14 in primary hepatocellular carcinoma,and to focus on their roles on the development, progress and metastasis of tumor. Methods 102 cases of primary hepato- cellular carcinoma were taken as the observation group and 80 cases of normal liver tissues as the control group. The expressions of Glypican3, MMP-9 and MMP-14 were detected by the immunohistochemistry method and their expression difference in different clinicopathological characteristics and the correlation were investigated. Results The positive rates of Glypican3, MMP-9 and MMP-14 expressions in the observation group were significantly higher than those in the control group. The positive rates of Glypi- can3, MMP-9 and MMP-14 expressions were closely correlated with the tumor volume,differentiation degree,lymph node metasta- sis,vessel infiltration, membrane invasion and PCNA expression. ＂][＇he correlation analysis showed that the positive relationships were found between Glypican3 and MMP-9 ,between Glypican3 and MMP-14 and between MMP-9 and MMP-14 in the observation group（r 0.48,P=0. 024 1;r--0.46,P=0. 013 2;r=O. 43. P=0. 031 3）. The survival analysis showed that expressions of Glypi- can3,MMP-9 and MMP-14 were correlated with the patient＇s prognosis. Conclusion The higher-expressions of Glypican3,MMP 9 and MMP-14 may promote the occurrence and development of primary hepatocellular carcinoma. Detecting the postoperative expres- sions of Glypican3, MMP-9 and MMP 14 has certqain value to judge the prognosis in primary hepatocellular carcinoma.

关 键 词：肝肿瘤 磷脂酰肌醇蛋白聚糖类 基质金属蛋白酶9 基质金属蛋白酶14 预后 

分 类 号：R735.7[医药卫生—肿瘤]