多药耐药相关蛋白1在人肿瘤细胞系K562、A549、SGC7901和SGC7901／VCR的表达  

Expression of multidrug resistance associated protein 1 in human tumor cells K562, A549, SGC7901 and SGC7901/VCR

作　　者：曹兴玥[1] 靳艳[1] 路军秀[1] 王国栋[2] 高福莲[3] 

机构地区：[1]新乡医学院组织学与胚胎学教研室,新乡453003 [2]新乡医学院三全学院,新乡453003 [3]新乡医学院基础医学院,新乡453003

出　　处：《解剖学杂志》2013年第6期1049-1052,共4页Chinese Journal of Anatomy

摘　　要：目的：探讨多药耐药相关蛋白1（MRPl）在常见人肿瘤细胞的表达。方法：培养K562、A549、SCK；7901和SGC7901／VCR细胞，RT-PCR检测ABCClmRNA的表达；SABC法检测MRPl蛋白的表达。结果：4种细胞均有ABCClmRNA的表达，SCK27901／VCR细胞的ABCClmRNA水平显著高于SCX27901细胞；SGC7901／VCR细胞MRPl表达显著高于SGC7901。结论：ABCC1／MRP1可能参与SGC7901细胞获得性多药耐药。Objective： The expression of multidrug resistance associated protein 1 （MRP1） in common human tumor cells was investigated. Methods： K562, A549, SCa27901 and SGC7901/VCR cells were cultured. The expression of ABCC1 mRNA was detected by RT-PCR1 The expression of MRP1 was tested by immunoeytochemistry. Results： There were ABCC1 expression in K562, A549, SGC7901 and SCK27901/VCR cells. The expression level of ABCC1 mRNA in SGC7901/VCR cells was higher than that in SGC7901 cells. The average of optical density was （0.73±0.21）, （0.60±0.18）, （1.23±0.34）, （1.90±0.26） in K562, A549, SGC7901, SGC7901/VCR cells respectively. The expression level of MRP1 in SGC7901/VCR cells was higher than that in SGC7901 cells. Conclusion： The expression of ABCC1/MRP1 was found in K562, A549, SGC7901 and SGC7901/VCR cells. And there was ABCC1/MRP1 in acquired multidrug-resistance of SGC7901 cells.

关 键 词：多药耐药相关蛋白1 人肿瘤细胞系 

分 类 号：R735.2[医药卫生—肿瘤]