巨颌症致病基因SH3BP2突变体对成纤维细胞的增殖抑制作用研究  

Inhibition of Cherubism Mutant SH3BP2 on the Growth of Fibroblasts.

作　　者：许舒宇 李翠英[1] 徐欣[2] 王海丞[1] 杨月[1] 于世凤[1] 

机构地区：[1]北京大学口腔医院中心实验室,北京100081 [2]山东大学口腔医学院

出　　处：《口腔医学研究》2014年第1期5-8,12,共5页Journal of Oral Science Research

基　　金：北京市自然科学基金(编号:7092037)

摘　　要：目的:观察过表达突变型SH3BP2(p.Asp419Gly)的单核细胞对颌骨成纤维细胞增殖的影响,探讨巨颌症病灶内纤维异常增殖的机制。方法:构建巨颌症致病基因SH3BP2突变(c.1256A>G)载体,转染单核细胞系Raw264.7,筛选过表达稳定株;以过表达野生型SH3BP2的Raw264.7稳定株为对照。制备Raw264.7条件培养基,培养颌骨成纤维细胞并对比其生长状态。结果:成功建立过表达细胞系。突变型组条件培养基中成纤维细胞减少速率小于野生型组(P<0.05)。结论:与野生型相比,突变型SH3BP2单核细胞对颌骨成纤维细胞生长抑制作用较弱,可能是巨颌症病灶常见纤维异常增殖的原因之一。Objective： To investigate the effects of monocytes over--expresson mutant SH3BP2 （p. Asp419 Gly） on the fibroblasts isolated from mouse mandible, for the purpose of exploring the possible reason of fibrosis in cherubism. Methods： We established the eukaryotic expression vector of mutant SH3BP2 （c. 1256A^G）, which was transfected into the mus monocytes Raw264.7 to acquire the stable cell line over--expresson mutant SH3BP2 （p. Asp419 Gly）; the Raw264.7 over--expresson wild type SH3BP2 were taken as control. Then the conditioned medium was collected to culture the fibroblasts isolated from mouse mandible, with which the growth was analyzed. Results： The stable cell lines of Raw264. 7 over--expresson wild type or mutant （p. Asp419 Gly） SH3BP2 were successfully established; and in contrast with the fibroblast cultured with the conditioned medium from wild type groups, the lost rate of fibroblasts with mutant groups was significantly lower （P〈0.05） . Conclusion： Compared to the wild type, the monocytes over--expresson mutant （p. Asp419 Gly） SH3BP2 exhibited less inhibition on the growth of mandible fibroblasts. Perhaps it results in the frequent fibrosis in cherubism at least partly.

关 键 词：巨颌症 突变 成纤维样细胞 SH3BP2 条件培养基 

分 类 号：R782.2[医药卫生—口腔医学]