黑灵芝多糖对S-180荷瘤小鼠腹腔巨噬细胞cAMP/PKA、IP_3/Ca^(2+)及DAG/PKC信号通路的影响  

Effect of a Polysaccharide from Ganoderma atrum on cAMP/PKA,IP_3/Ca^(2+) and DAG/PKC Signaling Pathways in Peritoneal Macrophages of S-180 Tumor-Bearing Mice

作　　者：黄建琴[1] 聂少平[1] 张莘莘[1] 黄丹菲[1] 朱科学[1] 谢明勇[1] 

机构地区：[1]南昌大学食品科学与技术国家重点实验室,江西南昌330047

出　　处：《食品科学》2014年第1期204-208,共5页Food Science

基　　金：国家自然科学基金重点项目(31130041);国家自然科学基金项目(31071532);"十二五"国家科技支撑计划项目(2012BAD33B06);教育部"新世纪优秀人才支持计划"项目(NCET-12-0749)

摘　　要：目的:探讨黑灵芝多糖(polysaccharide from Ganoderma atrum,PSG-1)对S-180荷瘤小鼠腹腔巨噬细胞环磷酸腺苷(cyclic adenosine monophosphate,cAMP)/细胞蛋白激酶A(protein kinase A,PKA)、三磷酸肌醇(inositol triphosphate,IP3)/Ca2+及甘油二酯(diacylglycerol,DAG)/蛋白激酶C(protein kinase C,PKC)信号通路的影响。方法:将S-180细胞接种于BALB/c小鼠体内建立荷瘤小鼠模型,成模后收集腹腔巨噬细胞进行体外培养,用不同浓度的PSG-1干预;ELISA法分别检测巨噬细胞培养上清液中的IP3、DAG和AMP含量;流式细胞仪测定细胞内Ca2+含量;Western blotting测定细胞PKA及PKC蛋白表达。结果:PSG-1在20~160μg/mL质量浓度范围能促进S-180荷瘤小鼠腹腔巨噬细胞产生cAMP、IP3和DAG,同时能增加细胞内Ca2+含量,并显著增强PKA及PKC蛋白表达量。结论:PSG-1能有效激活S-180荷瘤小鼠腹腔巨噬细胞cAMP/PKA、IP3/Ca2+及DAG/PKC信号通路。由此推测,PSG-1可能通过cAMP/PKA、IP3/Ca2+及DAG/PKC信号通路促进S-180荷瘤小鼠腹腔巨噬细胞抗肿瘤作用。Objective： To explore the effects of a polysaccharide from Ganoderma atrum （PSG-1） on cyclic adenosine monophosphate （cAMP）/protein kinase A （PKA）, inositol triphosphate （IP3）/Ca2＋ and diacylglycerol （DAG）/protein kinase C （PKC） signaling pathways in peritoneal macrophages of S-180 tumor-bearing mice. Methods： A tumor-bearing mouse model was established by inoculating mouse sarcoma S-180 cells into BALB/c mice; peritoneal macrophages were collected from the S-180 tumor-bearing mice, then cultured in vitro and treated with PSG-1 at various concentrations. The IP3, DAG and cAMP levels in cell culture supernatant were measured by ELISA. The intracellular Ca2＋ content was assayed by a flow cytometric method. The PKA and PKC protein expression in macrophages was determined by Western blotting. Results： PSG-1 in the concentration range of 20-160 μg/mL stimulated the production of IP3, DAG and cAMP in the macrophages from S-180 tumor-bearing mice, increased the intracellular Ca2＋ content, and increased the protein expression of PKA and PKC. Conclusion： PSG-1 can exert anti-tumor activity in peritoneal macrophages of S-180 tumor-bearing mice through the activation of cAMP/PKA, IP3/Ca2＋ and DAG/PKC signaling pathways.

关 键 词：黑灵芝多糖 腹腔巨噬细胞 抗肿瘤 信号通路 

分 类 号：Q291[生物学—细胞生物学]