籼稻耐盐性与稻米品质性状的关联分析  

Correlation Analysis of Salt Tolerance and Grain Quality Traits in Indica Rice( Oryza sativa L. )

作　　者：肖文斐[1] 马华升[1] 陈文岳[1] 裘劼人[1] 童建新[1] 郑桂珍[1] 忻雅[1] 王淑珍[1] 方献平[1] 阮松林[1] 

机构地区：[1]杭州市农业科学研究院生物技术研究所,浙江杭州310024

出　　处：《核农学报》2013年第12期1938-1947,共10页Journal of Nuclear Agricultural Sciences

基　　金：浙江省自然科学基金杰出青年项目(LR12C13001);国家自然科学基金项目(31301392)

摘　　要：选用31个籼型栽培稻品种,分别置于不同浓度的NaCl(0、2、4、6、8、9g·L-1)溶液中在30℃下发芽培养,考察其种子发芽及幼苗生长情况;同时利用近红外光谱分析上述品种的稻米品质(直链淀粉含量、胶稠度、碱消值及蛋白质、氨基酸含量)。结果表明,随着NaCl溶液浓度的增加,31个供试籼稻品种的种子生活力、活力及幼苗生长受到不同程度抑制。不同品种的耐盐表型差异显著,其中密阳46及其衍生品种在芽期及幼苗期均有较好的耐盐表现。相关性分析表明水稻平均发芽时间与稻米直链淀粉含量(AC)呈极显著负相关,与胶稠度(GC)显著正相关;而水稻耐盐比率则与AC呈显著正相关,与GC负相关。此外,水稻在清水及盐胁迫处理下的发芽势,发芽率、发芽指数与稻米总蛋白及大部分氨基酸含量呈显著正相关,而8 g·L-1NaCl溶液处理的水稻耐盐比率及耐盐指数仅与稻米Cys含量呈显著负相关。因此,水稻耐盐性与稻米品质性状存在一定关联性,在选育耐盐水稻品种时需兼顾稻米品质性状。Salt tolerance and grain quality are two important target traits for rice breeding. Although lots of studies have been done to investigate salt tolerance or grain quality in rice, the correlation between these two traits is poorly understood. In this study, the germination and seedling growth rate of the seeds of 31 indica rice varieties were investigated under the stress of various salt concentrations. The results showed that seed viability, vigor and seedling growth rate of all the 31 varieties were inhibited by the salt stress, and the inhibition effect strengthened as the concentration of NaC1 increased. Varieties displayed significantly different salt tolerance. Miyang 46 and its derivative varieties were found to own the strongest tolerance at germination and seedling stages. Meanwhile, by using the near- infrared reflectance spectroscopy method, grain quality assay was conducted to measure the seed amylose content, gel consistency, alkali spreading value, total protein and amino acid content. Correlation analysis indicated that the mean germination time is highly negatively correlated amylose content （AC）, but positively correlated with gel consistency （GC）. Conversely, tolerance ratio showed a significant positive correlation with AC, and a negative correlation with GC. In addition, under both H20 and salt stress conditions, the germination energy, germination ratio and germination index showed significant positive correlations with the content of total protein and most of amino acids. More interestingly, 0nly the Cys content was found to be negative correlated with tolerance ratio and salt tolerance index when 8 g·L-1 NaC1 was applied. In summary, our study shed some light on the correlation between salt tolerance and grain quality traits in rice. Grain quality traits can be potentially used as markers in salt-tolerant rice varieties breeding.

关 键 词：水稻 种子活力 耐盐性 蒸煮品质 营养品质 

分 类 号：S511.21[农业科学—作物学]