核苷酸切除修复交叉互补基因1核苷酸还原酶M1亚单位和乳腺癌易感基因1mRNA表达与中晚期胃肠道恶性肿瘤化疗方案选择及疗效的关系  

Relationship between the mRNA expression of ERCCl,RRM1 and BRCA1 and the clinical efficacy and prognosis in advanced gastrointestinal cancer

作　　者：张丰林[1] 陶自坚[2] 吕剑剑[3] 蒋帮勤 陈双柒[1] 王亦然[1] 高尔云[1] 王慧[1] 张燕[1] 孙鹏[1] 陈丽琼[1] 

机构地区：[1]安徽医科大学马鞍山临床学院肿瘤内科,马鞍山243000 [2]安徽医科大学马鞍山临床学院病理科,马鞍山243000 [3]安徽医科大学马鞍山临床学院胸外科,马鞍山243000

出　　处：《中国肿瘤临床与康复》2014年第1期41-44,F0003,共5页Chinese Journal of Clinical Oncology and Rehabilitation

摘　　要：目的检测以铂类为基础联合化疗的中晚期胃肠道恶性肿瘤患者中核苷酸切除修复交叉互补基因1(ERCC1)、核苷酸还原酶M1亚单位(RRM1)和乳腺癌易感基因1(BRCA1)mRNA的表达,探讨其与化疗疗效及预后的关系。方法采用mRNA定量检测方法,检测21例以铂类为基础联合化疗的中晚期胃肠道恶性肿瘤患者组织标本中ERCC1、RRM1和BRCA1mRNA的表达,并分析表达状况与化疗疗效及预后之间的关系。结果 (1)中晚期胃肠道恶性肿瘤组织中ERCC1、RRM1和BRCA1 mRNA表达与临床分期无关(P>0.05)。(2)ERCC1mRNA低表达患者化疗后总有效率(50.0%)高于ERCCl mRNA高表达患者(9.1%,P<0.05)。(3)RRM1 mRNA低表达患者化疗后总有效率(41.7%)高于RRM1 mRNA高表达患者(0,P<0.05)。(4)BRCA1 mRNA低表达患者化疗后总有效率(69.2%)高于BRCAl mRNA高表达患者(25.0%,P<0.05)。结论 ERCCl、RRM1和BRCA1mRNA表达水平,对中晚期胃肠道恶性肿瘤患者的化疗疗效及预后具有预测价值。Objective To investigate the relationship between the mRNA expression of ERCC1, RRM1 and BRCA1 and the clinical efficacy, prognosis in advanced gastrointestinal cancers treated by platinum-based chemotherapy. Methods Detected mRNA expression of ERCC1, RRMI and BRCA1 by quantitative detection method in 21 patients. The relation between mRNA expression and efficacy, prognosis was analyzed. Results （ 1 ） There is no significant differences between mRNA expression of ERCC1, RRM1, BRCA1 and clinical stage（ TNM stage） of advanced gastrointestinal cancers （ P 〉 0.05 ）. （2） The efficacy of chemotherapy（ ORR） in the low ERCC1 mRNA expression group was better than that in the high ERCC1 mRNA expression group （50. 0% vs. 9. 1%, P 〈0.05）. （3） There was significant difference in ORR between the low RRM1 mRNA expression group and the high RRM1 mRNA expression group. The efficacy of ORR in the low RRM1 mRNA expression group was better than that control （41.7% vs 0, P 〈0.05）. （4）The efficacy of ORR in the low BRCA1 mRNA expression group was better than that in the high BRCA1 mR- NA expression group （69. 2% vs 25.0%, P 〈 0. 05）, respectively. Conclusions Multi-biomarker （ER- CC1, RRM1 and BRCA1 ） detection may provide important predictive value for chemotherapy efficacy and prognosis in advanced gastrointestinal cancers.

关 键 词：胃肿瘤 肿瘤标记物 基因检测 药物疗法 治疗结果 预后 

分 类 号：R737.9[医药卫生—肿瘤]