5种小檗科植物多糖含量及抗氧化活性分析  

Content and Antioxidant Activities of Polysaccharides in 5 Species of the Family of Berberidaceae

作　　者：张华峰[1] 李璐[1] 张翔[1] 杨晓华[2] 姚美[1] 

机构地区：[1]药用资源与天然药物化学教育部重点实验室西北濒危药材资源开发国家工程实验室陕西师范大学食品工程与营养科学学院,陕西西安710062 [2]卫生部法医学重点实验室西安交通大学医学院,陕西西安710061

出　　处：《精细化工》2014年第2期174-178,共5页Fine Chemicals

基　　金：中央高校基本科研业务费专项资金项目(GK201302048);陕西省大学生创新创业训练计划项目(cx13063)~~

摘　　要：应用苯酚-硫酸比色法测定了南天竹(Nandina domestica)、日本绿叶小檗(Berberis thunbergii)、红叶小檗(Berberis thunbergii var.atropurpurea)、十大功劳(Mahonia fortunei)和阔叶十大功劳(Mahonia bealei)的多糖含量,应用ABTS[2,2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐]和DPPH(1,1-二苯基-2-三硝基苯肼)方法分析了5种小檗科植物多糖的抗氧化活性。结果表明,南天竹、日本绿叶小檗、红叶小檗、十大功劳和阔叶十大功劳的多糖含量平均值分别为27.82%、14.52%、12.14%、14.44%和16.80%,清除ABTS·+自由基的EC50值分别为0.168 8、0.543 6、0.160 4、0.399 1和0.168 9 g/L,清除DPPH自由基的EC50值分别为0.067 8、0.376 9、0.210 4、0.291 7和0.222 4 g/L。相同属植物多糖的含量无显著差异,不同种植物多糖的含量及抗氧化活性差异较大。多糖样品的抗氧化活性存在明显的剂量依赖性,基于量效关系建立的数学模型具有较高的拟合度。南天竹、阔叶十大功劳多糖含量较高且抗氧化活性较强,具有一定的开发利用价值。The content of polysaccharides in five species of the family of Berberidaceae, including Nandina domestica, Berberis thunbergii, Berberis thunbergii var. atropurpurea, Mahonia fortunei and Mahonia bealei, was determined by phenol-sulfuric acid method, and the antioxidant activities of polysaccharides in these five species were evaluated by ABTS [ 2,2＇-azino-bis （3-ethyl-benzothiazoline- 6-sulfonic acid） diammonium salt and DPPH （ 1,1-diphenyl-2-picrylhydrazyl ） assays. The average content of polysaccharides in Nandina domestica, Berberis thunbergii, Berberis thunbergii var. atropurpurea,Mahonia fortunei and Mahonia bealei was 27.82%, 14. 52%, 12. 14% , 14.44% and 16. 80% ,respectively. EC50 values of ABTSradical-scavenging capacity were 0. 168 8,0. 543 6, 0. 160 4,0.399 1 and 0. 168 9 g/L,respectively,and EC50 values of DPPH radical-scavenging capacity were 0.067 8,0. 376 9,0. 210 4,0. 291 7 and 0. 222 4 g/L, respectively. There is no significant difference in polysaccharide content among the same genera, while polysaccharide content and antioxidant activities are different among various species. Both ABTS and DPPH radical-scavengingcapacities were dose-dependent, and the mathematical model based on dose-effect relationship had goodness of fit. The content and antioxidant activities of polysaccharides in Nandina domestica Mahonia bealei were relatively high, which shows great potential for exploitation and utilization.

关 键 词：多糖 抗氧化活性 小檗科植物 纯化 中药现代化技术 

分 类 号：R282.7[医药卫生—中药学]