考试压力对大学生唾液中CCL28与sIgA的影响  

Effect of academic stress on CCL28 and sIgA in saliva of undergraduates1

作　　者：戴美璐 肖孟[1] 赵兵杰[1] 张蒙蒙[1] 姜广水[1] 

机构地区：[1]山东大学口腔医学院综合科山东省口腔生物医学重点实验室,济南250012

出　　处：《山东大学学报（医学版）》2014年第1期100-103,108,共5页Journal of Shandong University:Health Sciences

基　　金：2011年度国家级大学生创新创业训练计划项目(1110422067)

摘　　要：目的通过检测大学生考试前后唾液中黏膜相关上皮趋化因子(mucosa-associated epithelia chemokine,CCL28/MEC)与分泌型免疫球蛋白A(secretory immunoglobin A,sIgA)含量的变化,探讨考试应激对口腔黏膜免疫功能的影响。方法收集37名大学生(17名大二女生,10名大三男生和10名大三女生,后两者分别设为男生组和女生组)考试前后的非刺激性全唾液,采用酶联免疫吸附试验检测唾液中CCL28与sIgA水平的变化。结果考后37名学生唾液中CCL28与sIgA的吸光度值低于考前水平,差异有统计学意义(P<0.05);女生组比男生组的考试前后唾液CCL28水平的变化更显著(P<0.05);女生组考试前后唾液中CCL28水平的变化比sIgA更为明显(P<0.05),而男生组考试前后唾液中两指标间变化的差异不明显。结论考试应激能促进唾液中sIgA与CCL28水平的升高,女性比男性更容易受考试应激的影响。Objective To investigate the effect of academic stress on oral mucosal immune function by semi-quantitatively detecting and comparing the levels of mucosa-associated epithelia chemokine （CCL28/MEC） and secretory immunoglobin A （slgA） in saliva before and after certain exams. Methods A total of 37 undergraduates （ 17 sophomore girls, 10 junior boys and 10 junior girls, the latter two were defined as the male group and the female group ） were en- rolled to collect non-stimulated completed saliva before and after certain exams. The changes of CCL28 and slgA in the samples were detected with ELISA. Results The OD values of slgA and CCL28 after exams significantly decreased （ P 〈 0.05 ） in all 37 students. The change of CCL28 （P 〈 0.05 ） in the saliva spanning the exams was more significant in the female group than in the male group, whereas the change of slgA level （ P 〈 0.05 ） showed no obvious differ- ence. After the same exam, the change of CCL28 in saliva was more obvious than the change of slgA in the female group （ P 〈 0.05）, without significant difference in the male group （P 〉 0.05 ）. Conclusion Academic stress plays an important role in the secretion of CCL28 and slgA in saliva, and females are more susceptible to such stress.

关 键 词：唾液 分泌型免疫球蛋白A 黏膜相关上皮趋化因子 考试应激 

分 类 号：R781.4[医药卫生—口腔医学]