慢性牙周炎患者龈下菌斑的厌氧菌RFLP分型分析  

An Analysis on Subgingival Plaque in Patients with Chronic Periodontitis Anaerobic Bacteria RFLP Typing

作　　者：吴江华[1] 马校卫[2] 黄祖新[2] 

机构地区：[1]福建师范大学医院口腔科,福建福州350001 [2]福建师范大学生命科学学院,福建福州350108

出　　处：《武夷学院学报》2013年第5期105-109,共5页Journal of Wuyi University

摘　　要：目的:用PCR-RFLP技术,对慢性牙周炎患者龈下菌斑中厌氧菌进行分型。为建立更为合理的分型方法提供依据。方法:选取符合标准的慢性牙周炎患者,提取其龈下菌斑。厌氧条件下分离培养,获得厌氧菌菌株,在血平板培养基培养观察菌落的形态,通过染色观察菌株形态学特征,提取各菌株DNA,利用细菌16sDNA通用引物进行PCR扩增,以限制性内切酶MSP I对分离的22株厌氧菌的PCR扩增产物进行RFLP分型分析。结果:慢性牙周炎患者龈下菌斑中厌氧菌分为二大类。结论:采用PCR-RFLP用于厌氧菌的分型研究较为可靠,对于确定有争议菌种的分类具有重要的意义。Select compliant patients with chronic periodontitis, subgingiva/plaque was extracted. Cultured under anaerobic conditions, 22 anaerobes were picked Observe colony morphology in Blood Agar Plate. Through the dyeing morphological extracted DNA of every anaerobes and use thel6sDNA general primer for PCR amplification, the PCR products of the 22 pieces of bacteria are cut by restriction enzymes MSP I to RFLP Polymorphism analysis, the digestion of MSP I divide the 22 anaerobes into two categories.

关 键 词：牙周炎 厌氧菌 

分 类 号：R781.4[医药卫生—口腔医学]