麦芽中原花青素的提取纯化  

Extraction and purification of procyanidins from malt

作　　者：李玉妹[1,2] 刘春凤[1,2] 李崎[1,2] 

机构地区：[1]江南大学教育部工业生物技术重点实验室,江苏无锡214122 [2]江南大学酿酒科学与工程研究室,江苏无锡214122

出　　处：《食品与发酵工业》2014年第1期244-249,共6页Food and Fermentation Industries

基　　金：江苏高校优势学科建设工程资助项目(PAPD);国家创新基金(09C26213203751);江苏省创新基金(BC2009291);111引智计划;教育部新世纪人才支持计划项目资助(No.NCET-10-0453);国家高技术研究发展计划(863计划;No.2012AA021303);国家高技术研究发展计划(863计划;No.2013AA102106-03);国家自然科学基金(31271919)

摘　　要：为更好地研究麦芽来源的原花青素性质,建立了麦芽中原花青素的提取方法,用AB-8大孔吸附树脂对提取液分离纯化,进行动态吸附解吸实验,得到以乙醇为萃取剂的有机溶剂萃取佳条件为:乙醇体积分数60%,提取温度85℃(乙醇处于沸腾状态),料液比为1∶25(g∶mL),提取时间1.5 h。AB-8大孔吸附树脂动态吸附条件:样品质量浓度0.57 mg/mL,上样流速0.5 mL/min。动态解吸条件:洗脱剂乙醇体积分数60%,洗脱流速为1 mL/min,洗脱剂体积为65 mL,麦芽原花青素回收率可达95.2%,干基含量(纯度)为67.58%。In order to investigate the properties of procyanidins from malt,extraction and purification methods of procyanidins were established.The crude procyanidins from malt were purified by AB-8 macroreticular resin,and the purification conditions were researched through adsorption and desorption experiment.The optimum conditions of organic solvents extraction were as follows： 60% ethanol（ v/v） as the solvent,extraction temperature 85 ℃,the ratio of solvent to material at 25∶1（ g∶mL）,extraction time 1.5 h.The most suitable adsorption conditions were： sample concentration 0.57 mg/mL,and flow velocity 0.5 mL/min.And the desorption conditions were： 60% ethanol（v/v）,flow rate 1 mL/min,elution volume 65 mL.The recovery efficiency can be reached to 95.2 %,and the purity was 67.58%.

关 键 词：麦芽 原花青素 大孔树脂 提取 

分 类 号：TQ914[化学工程]