垂丝海棠对α-葡萄糖苷酶的抑制活性  

α-Glucosidase Inhibitory Activity of Malus halliana Koehne

作　　者：张伟[1] 常美芳[2] 金靖宜 申乾[2] 王军杰[2] 康文艺[1,2] 

机构地区：[1]黄河科技学院,郑州450063 [2]河南大学天然药物研究所,河南开封475004

出　　处：《中国实验方剂学杂志》2014年第4期84-86,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：河南省教育厅科学技术研究重点项目(2014年度);郑州市科技局重点攻关项目(20131015);河南大学大学生创新性实验计划项目(14B360011)

摘　　要：目的:对垂丝海棠花和叶α-葡萄糖苷酶抑制活性进行研究。方法:利用体外α-葡萄糖苷酶抑制模型,以阿卡波糖为阳性对照对α-葡萄糖苷酶抑制活性评价。结果:垂丝海棠花和叶的不同提取部位均具有一定的α-葡萄糖苷酶抑制活性,垂丝海棠叶70%乙醇部位(IC50=69.68 mg·L-1)的α-葡萄糖苷酶的抑制活性最好,远高于阳性对照阿卡波糖(IC50=1 213.38 mg·L-1)。垂丝海棠叶中70%乙醇部位、石油醚部位和乙酸乙酯部位活性均高于垂丝海棠花中相应部位,而叶中正丁醇部位略低于花的正丁醇部位,且都高于阳性对照阿卡波糖。此外,各部位的抑制活性均与质量浓度呈现相关性,具有一定的浓度依赖性。结论:垂丝海棠花和叶不同提取部位均具有一定的α-葡萄糖苷酶抑制活性,但不同提取部位其抑制活性有差别。Objective： To evaluate the α-glucosidase inhibitory activity of the flowers and leaves of Malus halliana Koehne. Method： By used α-glucosidase inhibitory model in vitro and with acarbose as positive control, the a-glucosidase inhibitory activity of the flowers and leaves of M. halliana were evaluated. Result： Different extracts from the flowers and leaves of M. halliana all had inhibitory activity of α-glucosidase, among which leaves of M. halliana 70% ethanol extract （IC50=69.68 mg.L-1） had the highest inhibitory activity ofα-glucosidase, it was far higher than that of the positive control acarbose （ IC50 = 1 213.38 mg -L-1 ）. 70% Ethanol extract, petroleum ether extract and ethyl acetate extract of leaves of M. halliana were higher than that of corresponding extract of flowers of M. halliana, while n-butanol extract of leaves was lower than that of n-butanol extract of flowers, and they were all higher than that of acarbose. In addition, the concentration of all extracts showed positive correlation with inhibitory activity, and also showed a certain degree of concentration dependence. Conclusion： Different extracts from the flowers and leaves of M. halliana all had inhibitory activity of α-glucosidase, but different extracts of the inhibition rate had certain difference.

关 键 词：垂丝海棠 提取部位 Α-葡萄糖苷酶 抑制活性 

分 类 号：R284.1[医药卫生—中药学]