1株产纤维素酶菌株的筛选及酶学性质的研究  

Selection and Enzymatic Properties of Cellulase-producing Strain

作　　者：李洁[1] 何兵[2] 许鹏[2] 雷波[1] 

机构地区：[1]四川省农业科学院农业信息与农村经济研究所,四川成都610066 [2]四川师范大学生命科学学院,四川成都610066

出　　处：《贵州农业科学》2013年第12期123-126,共4页Guizhou Agricultural Sciences

基　　金：四川省教育厅面上项目"农业有机废物堆肥发酵技术改良研究"(11ZB077)

摘　　要：为了进一步明确产纤维素菌在生产上的利用价值，拓展纤维素酶生产菌株的筛选渠道，采用CMC平板筛选和刚果红染色法，从四川师范大学成龙校区竹林地土壤中筛选获得1株产纤维素酶的菌株，编号为7F1，用聚合酶链式反应（PCR）扩增出菌株7F1的16SrDNA全基因序列，并对其进行测序，用blast软件在Genbank中对获取的序列进行同源性检索，并利用MEGA5．0软件构建系统发育树。结果表明：7F1菌株为B．aryabhattai，对菌株所产纤维素酶进行酶学性质的研究发现，该酶的酶促反应最佳作用温度是40～50℃，最佳作用pH6．0～8．0，该菌株对纤维素有较好的降解效果。说明，菌株7F1具有潜在的工业应用价值。To further define the utilization value of strain prcducing cellulose in production. A strain producing cellulase,coded as 7F1 ,was selected out from the soil in bamboo forest land in Sichuan Normal University by CMC plate selection and Congo red method. The 16SrDNA complete genome sequence o{ 7F1 strain was amplified by PCR and was sequenced. The homology blast of the 16SrDNA complete genome sequence from Genbank was operated by blast software and the phylogenetic tree of 16SrDNA was constructed by MEGAS. 0 software. Results： Based on phylogenetic tree, 7F1 strain should belong to B. aryabhattai. The enzymology properties study showed that the optimum temperature of enzymatic reaction was 40～ 50℃ and the optimum pH of enzymatic reaction was pH 6. 0～ 8.0. Conclusion： 7F1 strain had potential application value in industry.

关 键 词：纤维素酶 芽孢杆菌科 16 SrDNA 酶学特性 

分 类 号：S154.39[农业科学—土壤学]