驯化树干毕赤酵母发酵产乙醇的研究  

The Study of Ethanol Production by the Adapted Pichia stipitis

作　　者：陆青山[1] 韦策[1,2] 徐勇[1] 勇强[1] 余世袁[1] 

机构地区：[1]南京林业大学江苏省生物质绿色燃料与化学品重点实验室,江苏南京210037 [2]南京工业大学制药与生物工程学院,江苏南京211816

出　　处：《纤维素科学与技术》2013年第4期37-42,共6页Journal of Cellulose Science and Technology

基　　金：国家自然科学基金项目(31070523);江苏省科技支撑计划项目(BE2010732);林业公益性行业科研专项(201004001)

摘　　要：以玉米秸秆蒸汽爆破液为底物培养基,通过逐步提浓的方式对树干毕赤酵母菌株(Pichia stipitis)NLP23进行耐抑制物驯化,驯化后的菌株对汽爆液中甲酸和乙酸等抑制物的耐受能力可分别达到2.70 g/L和3.54 g/L,较出发菌株分别提高253.40%和277.80%。在含有57.34 g/L木糖和13.84 g/L葡萄糖的汽爆液中发酵42 h,糖利用率和乙醇得率可达到97.89%和65.83%,乙醇质量浓度为21.56 g/L,同时生成4.16 g/L木糖醇。玉米秸秆蒸汽爆破液中含有多种抑制酵母生长和发酵的有毒物质,主要是甲酸、乙酸、乙酰丙酸、糠醛和羟甲基糠醛,其中甲酸和乙酸含量较高,是影响树干毕赤酵母NLP23发酵汽爆液的主要抑制物。Corn stalks steam explosion of liquid has been utilized as a substrate for ethanol production using Pichia stipitis NLP23. Adaptation of the yeast was performed by sequentially transferring and growing cells in media containing increasing concentrations of Corn stalks steam explosion of liquid. The subculturing was carried out several times to obtain adapted strain. The adapted strain is finally able to tolerate higher inhibitor concentration, such as formic acid and acetic acid inhibitor-resistant ability can be reach 2.70 g/L and 3.54 g/L simultaneously, higher than the starting strain 277.80% and 253.40% separate. Main in the culture medium containing glucose 13.84 g/L, D-xylose 57.34 g/L, when, after 42 h fermentation, sugar utilization and ethanol yield can reach 97.89% and 65.83% respectively, while generating xylitol 4.16 g/L and ethanol 21.56 g/L respectively in fermentation broth. Corn stalks steam explosion fluid contains a number toxic substances which inhibit the growth and fermentation of yeast, mainly formic acid, acetic acid, levulinic acid, furfural and hydroxymethyl furfural, in which formic acid and acetic acid are the main inhibitors impact on fermentation of yeast E stipitisNLP23 because of their higher levels.

关 键 词：乙醇发酵 抑制物 树干毕赤酵母 驯化 

分 类 号：TQ92[轻工技术与工程—发酵工程] TQ353.42