都匀楼梯草总黄酮提取工艺及其抗氧化性能研究  

Study on extracting technology and Antioxidant Activity Total Flavonoids From Elatostema duyunens

作　　者：杨再波[1] 龙成梅[1] 毛海立[1] 孙成斌[1] 

机构地区：[1]黔南民族师范学院化学与化工系,贵州都匀558000

出　　处：《黔南民族师范学院学报》2013年第6期7-9,6,共4页Journal of Qiannan Normal University for Nationalities

基　　金：贵州省教育厅2010年度自然科学研究基金资助项目(黔教科2010094)

摘　　要：采用正交试验法优化都匀楼梯草中总黄酮的超声波提取工艺,考察超声波作用时间、乙醇浓度和液料比3个因素对都匀楼梯草总黄酮提取率的影响,确定都匀楼梯草总黄酮的优化超声波提取工艺条件为:提取溶剂80%乙醇,料液比1:25,超声波作用时间40 min,最佳提取率为1.62%。乙醇提取物抑制DPPH自由基的能力IC50=125.25μg/mL,说明都匀楼梯草不同极性部位均具有一定的抗氧化活性。Ultrasonic extraction of flavonoids in Elatostema duyunens was optimized by an orthogonal experiment. The effects of ultrasonic extraction treatment time, ethanol and ratio of liquor to material on the extraction yield of Elatostema duyunens were investigated. The optimized ultrasonic extraction was determined as follows： ratio of liquor to material 1：25 for 40 rain with 80% ethanol in a single ultrasonic extraction process ,The best extraction yield was 1.62%. DPPH radical inhibition capacity of ICso was 125.25μ g/mL, The results showed that the E. duyunens has some antioxidant activity.

关 键 词：都匀楼梯草 总黄酮 DPPH自由基 抗氧化性能 

分 类 号：TS272[农业科学—茶叶生产加工]