铁皮石斛多糖分离纯化及单糖组成测定  

Dendrobium polysaccharide purification and determination of monosaccharide composition

作　　者：宾宇波[1,2] 王亚芸[1,2] 安欣[1,2] 任建武[1,2] 

机构地区：[1]北京林业大学生物科学与技术学院食品科学与工程系,北京100083 [2]林业食品加工与安全北京市重点实验室(北京林业大学),北京100083

出　　处：《食品工业科技》2014年第4期122-125,共4页Science and Technology of Food Industry

基　　金：北京市园林绿化局计划项目(Y1HH200900304)

摘　　要：将铁皮石斛粗多糖经DEAE-52纤维素柱分离得到了四个多糖组分(DO-1、DO-2、DO-3、DO-4),并将组分DO-1经Sephadex G-200柱纯化得到多糖DOP。采用1-苯基-3-甲基-5-吡唑啉酮(PMP)柱前衍生化方法进行高效液相色谱分析,分析结果得出,多糖DOP由D-甘露糖、L(+)-鼠李糖和D-葡萄糖三种单糖组成,通过外标法定量得知D-甘露糖∶L(+)-鼠李糖∶D-葡萄糖=1.936∶0.856∶0.691。测定了多糖DOP抗氧化活性,结果显示其具有良好的清除能力。Polysaccharides of Oendrobium was isolated by OEAE - 52 cellulose column and four kinds of polysaccharide （DO-1 ,DO-2,DO-3,DO-4） were got, then the component DO-1 was purified by Sephadex G-200 column, polysaccharide OOP was got. Using the method of pre-column derivatization with 1-phenyl- 3 - methyl- 5 - pyrazolone （ PMP） for HPLC analysis and the results showed that, polysaccharides OOP was composed of D - man nose , L （ ＋ ） - rhamnose and D - glucose, and the external standard informed that D- man nose ： L （ ＋ ） - rhamnose： D - glucose = 1 .936： 0.856： 0.691. The antioxi dant properties of polysaccharide OOP were determined, and the results showed that the polysaccharide OOP had strong scavenging effects to OPPH radicals.

关 键 词：铁皮石斛 DEAE—cellulose-52纤维素柱 1-苯基-3-甲基-5-吡唑啉酮(PMP) 高效液相色谱 

分 类 号：TS201[轻工技术与工程—食品科学]