褪黑素对大鼠脊髓损伤后肿瘤坏死因子-α表达的影响  

Effects of melatonin on expression of TNF-α in rats with acute spinal cord injury

作　　者：徐玉生[1] 张松[1] 金伟林[1] 李星晨[1] 崔浩[1] 王培松[1] 文建国 

机构地区：[1]郑州大学第一附属医院骨科,郑州450052 [2]河南省高等学校临床医学重点学科开放实验室,郑州450052

出　　处：《郑州大学学报（医学版）》2014年第1期41-44,共4页Journal of Zhengzhou University(Medical Sciences)

基　　金：郑州市科技攻关计划项目2010S0828

摘　　要：目的:探讨褪黑素(MT)对脊髓损伤(SCI)大鼠体内肿瘤坏死因子-α(TNF-α)表达的影响及可能机制。方法:108只SD大鼠随机分为3组。假手术(Sham)组仅行椎板切除不损伤脊髓,SCI组与SCI+MT组采用改良的Allen's法建立SCI模型;SCI+MT组术后10 min腹腔注射MT(100 mg/kg),SCI组注射等量体积分数5%乙醇。术后12 h各组选6只取脊髓,采用HE染色法和免疫组化法分别观察脊髓组织的病理变化和TNF-α蛋白的表达情况。术后1、12、24、48、72 h,各组分别取6只大鼠,用ELISA法检测血清TNF-α水平,RT-PCR法测定脊髓组织TNF-αmRNA的表达。结果:SCI+MT组病理改变较SCI组明显减轻。脊髓组织中TNF-α蛋白主要分布在神经元和胶质细胞的胞浆和胞核;3组TNF-α阳性细胞数差异有统计学意义(F=504.239,P<0.001),其中SCI组最高,SCI+MT组次之,Sham组最低。各时间点3组血清TNF-α水平和脊髓组织中TNF-αmRNA的表达水平差异有统计学意义(P均<0.05),其中SCI+MT组上述2指标低于SCI组,但仍高于Sham组(P<0.05)。结论:MT可能通过抑制TNF-α的表达水平减轻损伤脊髓的继发性炎症损伤。Aim：To investigate the effects of melatonin （ MT） on the expression of tumor necrosis factor-α（ TNF-α） in acute spinal cord injury （ SCI） rats.Methods：A total of 108 SD rats were randomly allocated into three groups：Sham group only accepted laminectomy without SCI , and SCI group and SCI ＋MT group used the modified Allen＆#39;s method to establish SCI model.100 mg/kg MT was given to the rats in SCI ＋MT group, and 5% ethanol, to SCI group at 10 min after the model establishment .At 12 h, spinal cord samples of 6 rats from each group were prepared for histological examination u-sing HE and immunohistochemical SP staining to detect the expression of TNF-α.At 1,12,24,48 and 72 h, 6 rats in each group were executed ,the serum level of TNF-αwas determined by ELISA , and the expression of TNF-αmRNA was detec-ted by RT-PCR.Results：Compared with SCI group ,the pathological changes in SCI ＋MT group were significantly allevia-ted.Immunohistochemical staining showed that TNF-αprotein in spinal cord tissue mainly located in the cytoplasm and nu-cleus of neurons and glial cells;SCI group was the highest ,SCI＋MT group followed , and Sham group was the lowest （ F=504.239,P＆lt;0.001）.At different time points, the serum level of TNF-αand the expression of TNF-αin spinal cord tissue in SCI＋MT group were significantly lower than those in SCI group , but still higher than those in Sham group （P＆lt;0.05）. Conclusion：MT may reduce secondary injury of SCI by inhibiting the expression of TNF-α.

关 键 词：脊髓损伤 褪黑素 肿瘤坏死因子-Α 大鼠 TUMOR NECROSIS factor-α 

分 类 号：R681.54[医药卫生—骨科学]