p53基因在甲状腺癌中的突变研究  

Study on the mutation of p53 gene in thyroid carcinoma

作　　者：宋毅[1] 王炼[2] 黄光琦[1] 袁淑兰[1] 

机构地区：[1]华西医科大学附属第一医院肿瘤研究所肿瘤中心,成都610041 [2]华西医科大学口腔医学院病理室,成都610041

出　　处：《四川肿瘤防治》2001年第1期8-10,共3页Sichuan Journal of Cancer Control

摘　　要：目的 :为探讨 p53基因突变与甲状腺癌的发生、发展及预后的关系。方法 :应用PCR单链构象多态性(SSCP)分析技术 ,对p53基因第7,8外显子突变进行了检测和分析。结果 :在37例甲状腺癌中有11例在第7.8外显子发生突变 ,突变率为29.9 %。p53基因突变在复发的患者中显著高于未复发的患者 ;p53基因突变与转移、组织学类型和分化状况无显著差异。结论Objective:To investigate the mutation of p53 gene in the carcinogensis of thyroid carcinomas and the correlation between the p53 gene mutation and clinicopathological characteristics of the cancer.Methods:Single stranded conformation polymorphism of PCR was used in detecting p53 gene point mutations in exons 7,8.Results:p53 gene mutations in exons 7 and 8 were found in 37 thyroid carcinoma patients (29.7%).The frequency of mutations was significantly higher in relapse group than those in no relapse group (P<0.01).No statistically significant differences in p53 mutation were found relating to metastasis,histological type and differentiation of the cancer (P>0.05).Conclusion:These data suggest that the mutation of p53 gene may play an important role in thyroid carcinoma and may be associated with the prognosis of thyroid carcinoma patients.

关 键 词：P53基因 基因突变 甲状腺癌 SSCP-PCR 

分 类 号：R736.1[医药卫生—肿瘤]