气相色谱法测定脂肪酶催化反应体系中的己酸乙酯  

Determination of ethyl hexanoate in lipase catalysis reaction system by gas chromatography

作　　者：罗少华[1] 熊泽[1] 邵伟[1] 郭军[1] 

机构地区：[1]三峡大学化学与生命科学学院,湖北宜昌443001

出　　处：《中国酿造》2014年第1期137-140,共4页China Brewing

基　　金：三峡大学化学与生命科学学院资助项目(HY1103)

摘　　要：建立测定脂肪酶催化合成的己酸乙酯含量的毛细管气相色谱方法。以乙酸正戊酯为内标物,采用AT.PEG-20M(30m×0.25mm×0.33μm)的极性石英毛细管柱,程序升温:起始柱温55℃,恒温1min,以10℃/min升温至70℃,恒温2min,再以20℃/min升温至180℃,保持5min;进样口温度200℃;采用氢焰离子化检测器,在温度220℃条件下进行测定。己酸乙酯的最低检测限为2.774μg/mL,相对标准偏差为0.97%,平均加标回收率为97.44%。该方法快速、准确、灵敏,可用于检测脂肪酶催化反应体系中己酸乙酯的含量。The content of ethyl hexanoate catalyzed by lipase was determined by capillary gas chromatography. In this study, polar quartz capillary column （AT.PEG-20M 30m×0.25mm×0.331μm） was used and the internal standard was amyl acetate. The detection conditions were as follows： the initial column temperature 55℃ kept for 1 rain, then the temperature increased to 70℃ at a rate of 10℃/min for 2 mins, and then the temperature in- creased to 180℃ at a rate of 20℃/min for 5 rains. The temperature of injection port was 200℃; and the temperature of flame ionization detector was 220℃. The lowest detectable limit of ethyl hexanoate by the GC method was 2.7741μg/mL, the relative standard deviation was 0.97%, the average re- covery rate of ethyl hexanoate reached 97.44%. The method was fast, accurate and sensitive, and it could be used for determination of ethyl hex- anoate catalyzed by lipase.

关 键 词：气相色谱 脂肪酶 己酸乙酯 

分 类 号：O657.7[理学—分析化学]