分光光度法测定食物中植酸的研究  

Studies on the Determination of Physic Acid in Foods by Spectrophotometry

作　　者：吕杰 李耀根[2] 肖志芳[2] 

机构地区：[1]广州市天河区卫生防疫站,510630 [2]华西医科大学,成都610041

出　　处：《中国卫生检验杂志》2000年第6期652-654,共3页Chinese Journal of Health Laboratory Technology

摘　　要：本文建立了分光光度法测定用过量Ｆｅ３＋与植酸反应后的上清液中剩余Ｆｅ３＋，从而间接测定食物中植酸含量的方法，对测试条件进行了系统的探讨。该方法的线性范围为 ０～８μｇ植酸／ｍｌ；相关系数为 ０． ９９９５，检出限为 ０． ０９６μｇ植酸／ｍｌ，相对标准偏差为０．７％～９．２％，１５种样品的加标回收率为９６．６％～１０６．０％，该法与消化测磷法的测定结果比较，差异无显著性。该法灵敏度高，结果准确可靠，操作简便。We have developed a method for determining physic acid contents in foods by spectrophotometry based in determing residual Fe3+ in supernatant after precipitation of physic acid with excessive Fe3+. The linear range of the method,the correlation coefficient,the detection limit,relative standard deviation and recovery are 0-8ug physic acid/ ml, 0. 9995, 0. 096μg physic acid/ml, 0. 7 % - 9. 2 % and 96. 6- 106. 0%,respectively,the reliability of the method was evaluated by comparison with the method based on phosphors analysis (P>0. 05). This method is sensitive,accurate,simple for analysis of physic acid in foods.

关 键 词：植酸 食物 分光光度法 测定 

分 类 号：R151.3[医药卫生—营养与食品卫生学]