建立稳定表达融合自杀基因CD/UPRT.UL49的CNE-2鼻咽癌细胞株  

Construction of nasopharyngeal carcinoma CNE-2 cell lines expressing stable fusion suicide gene CD/UPRT.UL49

作　　者：卿菁[1,2] 赵素萍[1] 蒋卫红[1] 章华[1] 

机构地区：[1]中南大学湘雅医院耳鼻咽喉科,长沙410008 [2]浙江省宁波市第一医院耳鼻咽喉科,浙江宁波315010

出　　处：《临床耳鼻咽喉头颈外科杂志》2014年第3期178-181,共4页Journal of Clinical Otorhinolaryngology Head And Neck Surgery

基　　金：国家自然科学基金(No:30672296)

摘　　要：目的:建立稳定表达融合自杀基因CD/UPRT.UL49的CNE-2鼻咽癌细胞株。方法:构建表达载体pcDNA3.1(-)E6.BARF1p.CD/UPRT.UL49,经脂质体法转染CNE-2细胞,G418和前体药物5-氟胞嘧啶筛选出阳性克隆细胞并扩大培养。抽提阳性克隆细胞总蛋白质,Western-blotting检测目的基因表达。结果:融合自杀基因CD/UPRT.UL49在CNE-2转染细胞内稳定表达。结论:本组通过脂质体转染技术,成功地建立了稳定表达融合CD/UPRT.UL49基因的CNE-2细胞株。Objective：To construct nasopharyngeal carcinoma CNE-2 cell lines expressing stable fusion suicide gene CD/UPRT. UIA9. Method：The plasmids of pcDNA3. 1 （一）E6. BARFlp. CD/UPRT. UIA9 was transfected into CNE-2 cells through lipofectamine, and the transfected CNE-2 cells were selected by G418 and prodrugs for getting the cells expressing fusion CD/UPRT. UIA9 gene. The protein produced by the suicide gene was tested by Western-blotting in CNE 2 cells. Result： Suicide genes were expressed stably in CNE-2 cells. Conclusion： We con- structed nasopharyngeal carcinoma cell lines CNE-2 expressing stable suicide gene through lipofectamin.

关 键 词：自杀基因 CD UPRT UI.49 CNE-2细胞 鼻咽肿瘤 

分 类 号：R739.63[医药卫生—肿瘤]