A non-invasive,rapid method to genotype late-onset Alzheimer's disease-related apolipoprotein E gene polymorphisms  

作　　者：Li Yi Ting Wu Wenyuan Luo Wen Zhou Jun Wu 

机构地区：[1]Department of Neurology,Peking University Shenzhen Hospital [2]Department of Radiology,Peking University Shenzhen Hospital

出　　处：《Neural Regeneration Research》2014年第1期69-75,共7页中国神经再生研究（英文版）

基　　金：supported by two grants from Science,Industry,Trade and Information Technology Commission of Shenzhen Municipality in China,grant No.201002063,JC20110518075 7A

摘　　要：The apolipoprotein E gene ε4 allele is considered a negative factor for neural regeneration in late-onset Alzheimer＇s disease cases. The aim of this study was to establish a non-invasive, rapid method to genotype apolipoprotein E gene polymorphisms. Genomic DNA from mouth swab specimens was extracted using magnetic nanoparticles, and genotyping was performed by real-time PCR using TaqMan-BHQ probes. Genotyping accuracy was validated by DNA se- quencing. Our results demonstrate 100% correlation to DNA sequencing, indicating reliability of our protocol. Thus, the method we have developed for apolipoprotein E genotyping is accurate and reliable, and also suitable for genotyping large samples, which may help determine the role of the apolipoprotein E ε4 allele in neural regeneration in late-onset Alzheimer＇s disease cases.The apolipoprotein E gene ε4 allele is considered a negative factor for neural regeneration in late-onset Alzheimer＇s disease cases. The aim of this study was to establish a non-invasive, rapid method to genotype apolipoprotein E gene polymorphisms. Genomic DNA from mouth swab specimens was extracted using magnetic nanoparticles, and genotyping was performed by real-time PCR using TaqMan-BHQ probes. Genotyping accuracy was validated by DNA se- quencing. Our results demonstrate 100% correlation to DNA sequencing, indicating reliability of our protocol. Thus, the method we have developed for apolipoprotein E genotyping is accurate and reliable, and also suitable for genotyping large samples, which may help determine the role of the apolipoprotein E ε4 allele in neural regeneration in late-onset Alzheimer＇s disease cases.

关 键 词：nerve regeneration NEURODEGENERATION late-onset Alzheimer＇s disease apolipoprotein Egene real-time PCR DNA sequencing risk factor ALLELE neural regeneration 

分 类 号：R749.16[医药卫生—神经病学与精神病学]