双色荧光原位杂交检测放疗后鼻咽癌患者外周血淋巴细胞染色体畸变  

CHROMOSOME ABERRATIONS ANALYSIS IN PERIPHERAL LYMPHOCYTES OF NASOPHARYNGEAL CARCINOMA PATIENTS TREATED WITH RADIOTHERAPY USING TWO-COLOR FLUORESCENCE IN SITU HYBRIDIZATION

作　　者：徐永忠[1] 郑斯英[1] 朱巍[1] 高锦声[2] 赵经涌[1] 

机构地区：[1]苏州医学院放射医学系,江苏苏州215007 [2]苏州医学院生物学教研室,江苏苏州215007

出　　处：《癌变．畸变．突变》2001年第1期13-16,共4页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：国防科工委基金资助!(Y5 5 732 6 2 )

摘　　要：目的 :评价辐射诱发的染色体畸变作为癌患风险评估指标的可行性。方法 :应用双色荧光原位杂交 (FISH)技术检测 10例放疗后及 6例放疗前鼻咽癌患者外周血淋巴细胞染色体畸变并与常规法比较。结果 :放疗后 1～ 10年 ,鼻咽癌患者淋巴细胞染色体易位率、双着丝粒体率仍显著高于放疗前对照组的相应数值 (P <0 .0 0 1)。患者的易位率约为双着丝粒体率的 3 .5倍 ,照后 3年以上患者染色体易位率与照后小于 3年患者的易位率之间差别无显著性 (P >0 .1) ,而照后 3年以上患者染色体双着丝粒体率显著低于照后小于 3年患者的双着丝粒体率 (P <0 .0 0 1)。 结论 :稳定性染色体畸变 (易位 )在照后相当长时间 (10年 )仍可保持较高百分率 ,有望成为癌患风险评估的生物标志。Purpose:To evaluate the probability of using chromosome aberrations induced by irradiation as a indicator of future cancer risk. Methods:Chromosome aberrations in peripheral blood lymphocytes of 10 irradiated and 6 nonirradiated nasopharyngeal carcinoma patients were studied by two-colour fluorescence in situ hybridization(FISH) and coventional analysis. Results:Both the mean translocation and dicentric frequencies in irradiated patients 1 to 10 years after exposure were still significantly higher than those in nonirradiated patients(P<0.001). For irradiated patients,the translocation frequencies were about 3.5-fold as high as dicentric frequencies. No significant difference was found between translocation frequencies in patients after exposure more than 3 years and that in patients after exposure less than 3 years(P>0.1),but the dicentric frequencies in patients after exposure more than 3 years were significantly lower than that in patients after exposure less than 3 years(P<0.001)Conclusion:The percentages of stable chromosome aberrations(translocations)are still higher even a long time (10 years)after exposure and is expected to be a re1evant biomarker of future cancer risk.

关 键 词：荧光原位杂交 放射治疗 染色体畸变 鼻咽癌 

分 类 号：R739.630.5[医药卫生—肿瘤]