Ameliorating effects of constituents from Cortex Acanthopanacis Radicis on memory impairment in mice induced by scopolamine  

作　　者：Youngjoo Nam Dongung Lee 

机构地区：[1]Division of Bioscience,Dongguk University

出　　处：《Journal of Traditional Chinese Medicine》2014年第1期57-62,共6页中医杂志（英文版）

摘　　要：OBJECTIVE:To investigate the effects of active constituents extracted from Cortex Acanthopanacis Radicison improving the impaired memory in mice models.METHODS:The mice models of memory impairment were established using scopolamine.Ameliorating effects of the fractions and constituents on scopolamine-induced memory impairment in vivo were investigated using passive avoidance and Morris water-maze task tests,and their anti-acetylcholinesterase(AChE)and antioxidant activities in vitro examined.The isolation of constituents was performed by chromatographic methods and their structures were identified on the basis of instrumental analysis.RESULTS:Among the fractions tested,ethylacetate fraction exhibited the anti-AChE activity(25.83%±0.23%)properly and excellent 2,2-diphenyl-1-picrylhydrazyl(DPPH)radical and superoxide anion scavenging capacity(87.50%±0.83%and 60.22%±0.43%,respectively).However,the methylene chloride fraction was much more active than the ethylacetate fraction in the passive avoidance task test(167.5%increase of step-through latency time)and Morris water-maze task test(33.3%decrease of es-cape latency time).Four constituents,β-sitosterol,stigmasterol,sesamin,and hyperin were isolated from the methylene chloride fraction,among them,hyperin showed anti-acetylcholinesterase and anti-oxidant activities remarkably.Moreover,hyperin exerted a potent effect(146±38)s on memory improvement in terms of passive avoidance task test compared with the reference compound tacrine(162±43)s at a dose of 2.5 mg/kg.CONCLUSION:Hyperin,a flavonoid glucoside isolated from Cortex Acanthopanacis Radicis,inhibited AChE activity and potently ameliorated scopolamine-induced memory impairment,and its action may be partially mediated by the acetylcholine-enhancing cholinergic nervous system.OBJECTIVE：To investigate the effects of active con- stituents extracted from Cortex Acanthopanacis Radicison improving the impaired memory in mice models. METHODS： The mice models of memory impair- ment were established using scopolamine. Amelio- rating effects of the fractions and constituents on scopolamine-induced memory impairment in vivo were investigated using passive avoidance and Morris water-maze task tests, and their anti-acetyl- cholinesterase（AChE） and antioxidant activities in vitro examined. The isolation of constituents was performed by chromatographic methods and their structures were identified on the basis of instru- mental analysis. RESULTS： Among the fractions tested, ethylacetate fraction exhibited the anti-AChE activity（25.83%± 0.23%） properly and excellent 2,2-diphenyl-1-picryl- hydrazyl（DPPH） radical and superoxide anion scav- enging capacity（87.50% ± 0.83% and 60.22% ± 0.43%, respectively）. However, the methylene chlo- ride fraction was much more active than the ethyl- acetate fraction in the passive avoidance task test（167.5% increase of step-through latency time） and Morris water-maze task test（33.3% decrease of es-cape latency time）. Four constituents, β-sitosterol, stigmasterol, sesamin, and hyperin were isolated from the methylene chloride fraction, among them, hyperin showed anti-acetylcholinesterase and anti-oxidant activities remarkably. Moreover, hyperin exerted a potent effect（146±38） s on mem- ory improvement in terms of passive avoidance task test compared with the reference compound tacrine（162±43） s at a dose of 2.5 mg/kg. CONCLUSION： Hyperin, a flavonoid glucoside iso- lated from Cortex Acanthopanacis Radicis, inhibited AChE activity and potently ameliorated scopol- amine-induced memory impairment, and its action may be partially mediated by the acetylcholine-en- hancing cholinergic nervous system.

关 键 词：Cortex Acanthopanacis Radicis Stigmas-terol SESAMIN HYPEROSIDE ACETYLCHOLINESTERASE 

分 类 号：R285.5[医药卫生—中药学]