高效液相法检测饮料中六种人工合成着色剂  

Determination of Synthetic Coloring Agent in Beverage by High Performance Liquid Chromatography

作　　者：徐罕琦[1,2] 余雯静[1,2] 

机构地区：[1]苏州出入境检验检疫局检验检验综合技术中心,江苏苏州215104 [2]苏州世标检测技术有限公司,江苏苏州215104

出　　处：《农产品加工（下）》2014年第1期73-74,共2页Farm Products Processing

摘　　要：建立使用高效液相色谱（HPLC）测定饮料中6种人工合成着色剂含量的方法。采用C18色谱柱进行分离，以甲醇和乙酸铵作为流动相进行梯度洗脱使柠檬黄、苋菜红、胭脂红、日落黄、诱惑红、亮蓝6种着色剂完全分离。结果显示，各组分RSD值小于1%，回收率为93.6%-99.8%。该法前处理简单、回收率高、操作简便，测定结果令人满意。This article establishes the method of determining 6 kinds of coloring agent in beverage by HPLC. Chromatographic separation is performed on C18 column with methanol - ammonium acetate as mobile phase and gradient elution to cause the lemon yellow, amaranth, carmine, sunset yellow, allura red, and bright blue 6 kind of coloring agents to separate completely. The result shows that various components RSD is smaller than 1%, the recovery rate is 93.6%-99.8%. The pretreatment of this method is simple, the recovery rate is high, and the determination result is satisfying.

关 键 词：高效液相色谱法 着色剂 饮料 

分 类 号：TS275[轻工技术与工程—农产品加工及贮藏工程]