葡萄糖发酵液D-核糖含量的高效液相色谱分析  

High Performance Liquid Chromatography Analysis of D-Ribose Content in Glucose Zymotic Fluid

作　　者：张津枫[1] 王健刚[2] 邓国才[1] 杨四海[1] 陈荣悌[1] 

机构地区：[1]南开大学化学系,天津300071 [2]天津市红十字医院,天津300130

出　　处：《高等学校化学学报》2001年第1期43-45,共3页Chemical Journal of Chinese Universities

摘　　要：The method has been put forward to analyze the content of D-ribose obtained from glucose zymotic fluid. D-ribose was determined by HPLC, on a Sugar PakⅠcolumn, by using a refractive index detector and a mobile phase of pure water. We have also studied the separation conditions of sugars. It has many advantages, such as rapid analysis, high sensitivity and high precision and good reproducibility. The recovery of D-ribose were 95.5%—104%, RSD were less than 1 5%, the detection limit of D-Ribose was 50 ng.The method has been put forward to analyze the content of D-ribose obtained from glucose zymotic fluid. D-ribose was determined by HPLC, on a Sugar PakⅠcolumn, by using a refractive index detector and a mobile phase of pure water. We have also studied the separation conditions of sugars. It has many advantages, such as rapid analysis, high sensitivity and high precision and good reproducibility. The recovery of D-ribose were 95.5%—104%, RSD were less than 1 5%, the detection limit of D-Ribose was 50 ng.

关 键 词：高效液相色谱法 D-核糖 葡萄糖 发酵液 分析 

分 类 号：TQ929[轻工技术与工程—发酵工程] O657.72[理学—分析化学]