用改装的Super-Spinner搅拌瓶连续灌流培养产凝血酶原CHO工程细胞  

Continuously Perfused Cultivation of Genetically-engineered CHO Cells Producing Prothrombin in a Modified Super-Spinner

作　　者：陈昭烈[1] Kai Iding Dirk Lütkemeyer JürgenLehmann 

机构地区：[1]军事医学科学院生物工程研究所,北京100071 [2]德国比勒菲尔德大学细胞培养技术研究所

出　　处：《生物工程学报》2001年第1期109-112,共4页Chinese Journal of Biotechnology

摘　　要：A Super\|Spinner was Modified by mounting a stainless steel filter(pore size 75 μm)to the impeller shaft to retain cells while fresh nutrient is perfused.Using Macroporous microcarrier Cytopore 1,continuously perfused cultivation of a recombinant CHO cell line,CHO2DS producing prothrombin was performed with the perfusion of a protein\|free medium DF6S.The cell retention rate was more than 90% during the 24 days continuously perfused cultivation.The viable cell density of CHO2DS and prothrombin concentration reached 4.62×10 6(cells.m/L) and 11.3(mg/L)respectively after 9 days culture.A Super\|Spinner was Modified by mounting a stainless steel filter(pore size 75 μm)to the impeller shaft to retain cells while fresh nutrient is perfused.Using Macroporous microcarrier Cytopore 1,continuously perfused cultivation of a recombinant CHO cell line,CHO2DS producing prothrombin was performed with the perfusion of a protein\|free medium DF6S.The cell retention rate was more than 90% during the 24 days continuously perfused cultivation.The viable cell density of CHO2DS and prothrombin concentration reached 4.62×10 6(cells.m/L) and 11.3(mg/L)respectively after 9 days culture.

关 键 词：连续灌流培养 CHO工程细胞 凝血酶原 动物细胞 细胞培养 

分 类 号：Q813.3[生物学—生物工程]