鼠李糖脂逆胶束体系中纤维素酶的后萃研究  

Study on the Backward Extraction of Cellulase in Rhamnolipid Reverse Micelles

作　　者：赵艳鸽[1,2] 袁兴中[1,2] 黄华军[1,2] 崔凯龙[1,2] 彭馨[1,2] 彭子原[1,2] 曾光明[1,2] 

机构地区：[1]湖南大学环境科学与工程学院,长沙410082 [2]环境生物与控制教育部重点实验室(湖南大学),长沙410082

出　　处：《环境科学》2014年第2期759-763,共5页Environmental Science

基　　金：国家自然科学基金项目(50978087)

摘　　要：采用生物表面活性剂鼠李糖脂(rhamnolipid,RL)构建RL/异辛烷/正己醇的逆胶束体系,并研究了该体系中纤维素酶后萃过程的影响因素.分别考察了后萃水相pH值、振荡时间、离子种类和强度以及添加短链醇对纤维素酶的后萃率和酶活回收率的影响.结果表明,后萃水相最佳pH值为7.0,振荡时间以30 min为最佳,后萃水相中离子强度以0.15 mol·L-1KCl最佳,正丁醇的最佳添加量为2%.在最佳实验条件下,纤维素酶的后萃率和酶活回收率分别可以达到76.22%和93.39%.生物表面活性剂RL构建的逆胶束体系对纤维素酶的后萃效果较佳,且RL具有高生物降解性,低临界胶束浓度等优点,应用前景广阔.This paper studied the backward extraction of cellulase in RL/isooctane/n-hexanol reverse micelles system. Several key parameters influencing the backward extraction efficiency and activity recovery of cellulase were investigated, including stripping aqueous pH, stripping time, salt type and ionic strength, and addition of alcohols. The experiment results indicated that the optimal parameter values as follows ： stripping aqueous pH 7.0, stripping time 30 min, 0. 15 mol·L - 1 of KC1, dosage of n-butanol 2%. Under above optimum conditions, the backward extraction efficiency and activity recovery were up to 76.22% and 93.39% , respectively. The backward extraction of cellulase using reverse micelles based on biosurfactant RL performs well. Furthermore, RL has many advantages such as high biodegradability, low critical micelle concentration, etc. The application prospects of RL reverse micelles ale extensive.

关 键 词：逆胶束 纤维素酶 后萃 生物表面活性剂 鼠李糖脂 

分 类 号：X172[环境科学与工程—环境科学]