茶叶提取物EGCG对B16小鼠黑素瘤细胞的影响  

Effect of EGCG Extracted from Tea on B16 Melanoma Cells

作　　者：曾亮[1,2,3] 马梦君[1] 官兴丽[3] 肖海军[3] 罗理勇[1,2] 

机构地区：[1]西南大学食品科学学院,重庆400715 [2]西南大学茶叶研究所,重庆400715 [3]大益茶业集团勐海茶业有限责任公司技术中心实验室,云南西双版纳666200

出　　处：《西南大学学报（自然科学版）》2014年第2期171-177,共7页Journal of Southwest University(Natural Science Edition)

基　　金：国家自然科学基金项目资助(31100500);重庆市科委自然科学基金计划资助(CSTC;2013jcyjA80021);重庆市科委自然科学基金计划资助(CSTC;2010BB1136)

摘　　要：采用倒置显微镜观察茶叶中提取物EGCG对B16小鼠黑素瘤细胞形态的影响,亚甲基蓝染色法测定EGCG对B16细胞增殖率的影响,L-多巴氧化法测定EGCG对B16细胞酪氨酸酶活力的影响,氢氧化钠裂解法测定EGCG对B16细胞黑色素生成量的影响.结果表明:EGCG作用24h,高浓度组(>60μmol)细胞胞膜融合现象明显;作用72h,高浓度组大部分细胞死亡.EGCG对B16细胞增殖率、酪氨酸酶活性、黑色素生成量均有显著抑制,呈明显的剂量效应关系,且EGCG抑制B16细胞增殖,IC50为33.99μmol.Inverted microscopy was used to observe the effects of EGCG on the morphology of B16 melano ma cells; and the methylene blue method, the L-DOPA oxidation method and the NaOH decomposition method were used, respectively, to study the effects of EGCG on B16 cell proliferation rate, tyrosinase ac tivity and melanogenesis. In the high EGCG concentration （〉60 μmol） group, a conspicuous phenomenon of cell membrane fusion appeared after 24 hour action, and most of the cells died after 72-hour action. EGCG significantly decreased cell proliferation rate, tyrosinase activity and melanogenesis of B16, which was obviously dose dependent. EGCG inhibited B16 cell proliferation, IC50 being 33.99μmol.

关 键 词：EGCG 黑色素 酪氨酸酶 B16小鼠黑素瘤细胞 

分 类 号：Q5[生物学—生物化学]