Functional tuning and expanding of myoglobin by rational protein design  

作　　者：LIN YingWu WANG JiangYun LU Yi 

机构地区：[1]School of Chemistry and Chemical Engineering, University of South China [2]Laboratory of Noncoding RNA,Institute of Biophysics, Chinese Academy of Sciences [3]Department of Chemistry,University of Illinois at Urbana-Champaign,Urbana

出　　处：《Science China Chemistry》2014年第3期346-355,共10页中国科学（化学英文版）

基　　金：supported by the National Natural Science Foundation of China(21101091,31370812);the Scientific Research Foundation for the Returned Overseas Chinese Scholars,Ministry of Education of China.J.Wang is supported by the National Basic Research Program of China(2010CB912301,2009CB82 5505);the National Natural Science Foundation of China(90913022);Y.Lu is supported by the US National Institute of Health(GM062211)

摘　　要：Rational protein design is a powerful strategy,not only for revealing the structure and function relationship of natural metalloproteins,but also for creating artificial metalloproteins with improved properties and functions.Myoglobin(Mb),a small heme protein created by nature with diverse functions,has been shown to be an ideal scaffold for rational protein design.The progress reviewed herein includes fine-tuning its native functions of O2binding and transport,peroxidase activity and nitrite reductase(NIR)activity,and rational expanding its functionalities to peroxygenase,heme-copper oxidase(HCO),nitric oxide reductase(NOR),as well as hydroxylamine reductase.These studies have enhanced our understanding of how metalloproteins work in nature,and provided insights for rational design of functional metalloproteins for practical applications in the future.Rational protein design is a powerful strategy,not only for revealing the structure and function relationship of natural metalloproteins,but also for creating artificial metalloproteins with improved properties and functions.Myoglobin（Mb）,a small heme protein created by nature with diverse functions,has been shown to be an ideal scaffold for rational protein design.The progress reviewed herein includes fine-tuning its native functions of O2binding and transport,peroxidase activity and nitrite reductase（NIR）activity,and rational expanding its functionalities to peroxygenase,heme-copper oxidase（HCO）,nitric oxide reductase（NOR）,as well as hydroxylamine reductase.These studies have enhanced our understanding of how metalloproteins work in nature,and provided insights for rational design of functional metalloproteins for practical applications in the future.

关 键 词：METALLOPROTEINS heme proteins protein design OXIDASE REDUCTASE 

分 类 号：Q51[生物学—生物化学] TU986.2[建筑科学—城市规划与设计]