胆囊癌组织中cyclinD_1,cdk4,p16,Rb表达及意义  

Expression of cyclin D_1, cdk4, p16 and Rb in gallbladder carcinoma and its significance

作　　者：杨竹林[1] 李永国[1] 钟德玝[1] 刘国利[1] 

机构地区：[1]湖南医科大学附属第二医院肝胆疾病研究室,长沙410011

出　　处：《中华肝胆外科杂志》2001年第2期84-86,共3页Chinese Journal of Hepatobiliary Surgery

摘　　要：目的　研究胆囊癌组织中cyclinD1,cdk4,p16和Rb的表达及其相互关系。方法　石蜡包埋切片ABC免疫组织化学法。结果　 40例胆囊癌cyclinD1,cdk4,p16和Rb阳性病例率分别为42 5 % ,47 5 % ,32 5 %和 37 5 % ;高分化、组织学分级Ⅰ级和未转移病例cyclinD1,cdk4,Rb阳性率明显低于低分化、组织学分级Ⅲ级和转移病例 ;cyclinD1表达与cdk4表达呈正相关系和与P16呈负相关系 ,P16表达与Rb表达和cdk4表达呈负相关系。结论　cyclinD1,cdk4,p16和Rb表达特征与胆囊癌发生发展、生物学行为及其预后有密切关系 ,胆囊癌的分子发病机制可能涉及cdk4/cyclinD1,Rb和p16负反馈调节通路的异常。Objective To detect the expression of cyclin D 1, cyclin dependent kinase 4 (cdk4), p16 and Rb in gallbladder carcinoma and explore their relationships. Methods The immunohistochemical method of avidin-biotin complex in paraffin embedded sections was used. Results The positive rates of cyclin D 1, cdk4, p16 and Rb in 40 cases of gallbladder carcinoma were 42.5%, 47.5%, 32.5% and 37.5%, respectively. The positive rates of cyclin D 1, cdk4 and Rb were significantly lower in cases of well differentiation, histological grade Ⅰ and without metastasis than in those of poor differentiation, histological grade Ⅲ and with metastasis. The positive correlation was found between the expression of cyclin D 1 and that of cdk4 while negative correlation was found among the expression of p16 and that of cyclin D 1/cdk4 and Rb in gallbladder carcinoma. Conclusions The expression of cyclin D 1, cdk4, p16 and Rb might be related to the carcinogenesis, development, biological behaviors and prognosis of gallbladder carcinoma. Abnormality in the negative feedback regulatory pathway of cyclin D 1/cdk4, Rb and p16 might be involved in molecular mechanism of gallbladder carcinoma.

关 键 词：胆囊肿瘤 癌基因 免疫组织化学 CYCLIN D1 CDK4 

分 类 号：R735.8[医药卫生—肿瘤] R730.231[医药卫生—临床医学]