β-环糊精对多菌灵的荧光增敏效应研究  

Investigation of Fluorescence Enhancement Effect of Carbendazim by β-Cyclodextrin

作　　者：姜慧明[1] 杨红[2] 

机构地区：[1]大连民族学院生命科学学院,辽宁大连116605 [2]大连民族学院环境与资源学院,辽宁大连116605

出　　处：《大连民族学院学报》2014年第1期5-9,共5页Journal of Dalian Nationalities University

基　　金：中央高校基本科研业务费专项资金资助项目(DC10040106);大连民族学院人才启动基金资助项目(0701-110002)

摘　　要：研究了β-环糊精和多菌灵在水相中形成的超分子体系的荧光光谱，得到了不同浓度和不同pH值溶液中客体分子的荧光光谱。研究发现，在水相中，β-环糊精和多菌灵形成了1：1的超分子体系，包合常数为3．2×10^2，与单体分子相比，空腔内多菌灵的荧光强度大大提高。β-环糊精的浓度为8×10^-4-5．6＋10^-3mol·L^-1时，空腔内多菌灵的荧光强度随着β-环糊精浓度的增加而增强。多菌灵的荧光强度在酸性水溶液中具有很强的增强效应，而在中性和碱性介质中，其荧光增强效应较为平缓。在pH=7．6的溶液中，多菌灵在β-环糊精空腔内的荧光强度达到最大。多菌灵在质量浓度为0．46—6．16ng·mL^-1范围内呈现良好的线性关系，回归方程为F=5．37C（ng·mL^-1）＋148．54，相关系数为0．9993。利用荧光增强效应，得到该超分子体系对多菌灵的检测限为60Pg·mL^-1，是已经报道检测限（4．78ng·mL^-1）的百分之一。In this paper, the fluorescent spectra of the supramolecular system formed between β - eyclodextrin and earbendazim in aqueous media is investigated, the spectra of guest molecule in different concentrations and different pH value solution are obtained. Results show that in aqueous media, β -cyclodextrin and carbendazim form a 1 ： 1 supramolecuar system, its complexation constant is 3.2 x 102. In comparison with the single guest molecule, the fluorescence strength of guest molecule inside the cavity of β-cyclodextrin is increased in a remarkable extent. The fluo- rescence strength of carbendazim is increasing with the increasing concentration ofβ - cyclodextrin between 8 ＊ 10-aM - 5.6 x 10-3M. The fluorescence enhancement effect of guest molecule is very high in acidic aqueous media, however, it decreases mildly in neutral and basic media, the highest fluorescent strength is found in a pH = 7.6 solution. Within the concentration 0.46ng/mL -6. 16 ng/mL, the fluorescence strength of carbendazim exhibits a good linear relationship, whose regression equation is F = 5.37C（ng/mL） ＋ 148.54, the correlation coefficient is 0.999 3. By means of fluorescence enhancement effect, the detection limit of carbendazim in the supramolecular system reaches 60 pg/mL, one percent of the published result （4.78 ng/mL）.

关 键 词：Β-环糊精 多菌灵 包合作用 荧光增强效应 检测限 

分 类 号：S481[农业科学—农药学]