响应面法优化野波罗蜜根皮总黄酮的微波提取工艺  

Optimization of microwave extraction of flavonoids from wild Jackfruit root barks by response surface methodology

作　　者：李海滨[1,2] 胡晓旭[1,3] 普开 谭伟[1,4] 杨敏[1,4] 王红斌[1,4] 

机构地区：[1]云南民族大学,民族药资源化学国家民委,教育部重点实验室,云南昆明650500 [2]云南民族大学校医院,云南昆明650500 [3]大理市农产品质量安全检测站,云南大理671000 [4]云南民族大学化学与生物技术学院,云南昆明650500

出　　处：《食品工业科技》2014年第6期226-229,234,共5页Science and Technology of Food Industry

基　　金：民族药资源化学国家民委-教育部重点实验室开放基金项目(MZY1122);云南省应用基础研究计划项目(2009CD100);云南民族大学绿色化学与功能材料研究省创新团队项目(2011HC008);云南省高校绿色化学新能源与材料科技创新团队项目(2011UY09);云南民族大学化生学院大学生创新性实验项目(2011HXZ01);云南民族大学化生学院2011年大学生(硕士组)创新性实验项目(11HXYJSZ1)

摘　　要：目的:运用响应面分析法优化野波罗蜜根皮总黄酮的微波提取工艺。方法:以野波罗蜜根皮为材料,采用微波提取法,提取野波罗蜜根皮总黄酮。在单因素实验的基础上,利用Box-Benhnken中心组合实验和响应面分析法,优选微波法提取野波罗蜜根皮总黄酮的工艺条件。结果:野波罗蜜根皮总黄酮的最佳提取工艺为:在60%的乙醇溶液中微波提取,液固比为52∶1mL/g,微波时间为2.2min;此条件下,野波罗蜜根皮总黄酮实际提取率为9.82%,预测值为9.80%。结论:运用响应面分析法优化得到的微波提取参数准确、可靠。Objective.These optimum conditions for the microwave extraction of flavonoids from wild jackfruit root barks were determined using response surface methodology. Methods ,,Wild jackfruit root barks as the materiats,flavonoids were extracted by microwave extraction method. On the basis of one-factor-at-a-time experiments,the process conditions of microwave extraction of flavonoids from wild jackfruit root barks were optimized by Box-Benhnken central composite test and response surface analysis method. Results.Results showed that these optimal extraction conditions were as follows..60% ethanol solution as extraction solvent, microwave extraction time of 2.2min, liquid-to-solid ratio of 52： l mL/g. Under these conditions,the actual extraction rate of fiavonoids from wild jackfruit root barks total was 9.82% in comparison with the predicted yield of 9.80%. Conclusion ..The parameter of microwave extraction was optimized by response surface methodology are accurate and reliable.

关 键 词：野波罗蜜根皮 总黄酮 微波提取 响应面分析 

分 类 号：TS201.1[轻工技术与工程—食品科学]