全自动固相萃取-高效液相色谱荧光法测定蜜饯中人工合成甜味剂对乙氧基苯脲含量  

Determination of Artificial Sweetener 4-Ethoxyphenylurea in Succade by Automatic Solid-phase Extraction and High Performance Chromatography with Fluorescence Method

作　　者：陈章捷 陈金凤 张艳燕 钟坚海 魏晶晶 

机构地区：[1]福建龙岩出入境检验检疫局,福建龙岩364000

出　　处：《中国调味品》2014年第3期112-114,共3页China Condiment

基　　金：福建省科技攻关重点项目(2010Y0001)

摘　　要：提出了高效液相色谱法测定蜜饯中人工合成甜味剂对乙氧基苯脲含量的方法。样品采用醋酸-醋酸铵缓冲液超声提取,全自动固相萃取仪净化,SB-C18反相色谱柱分离,荧光检测器检测。对乙氧基苯脲在0-10 mg/L范围内的线性相关系数为0.9987,方法定量限（S/N=10）小于0.1 mg/kg。以三种空白蜜饯为基体,在3个添加水平进行加标回收试验,平均回收率在81.7%-92.4%之间,相对标准偏差（n=6）在2.4%-6.8%之间。High performance liquid chromatography is applied for the determination of artificial sweete-ner 4-Ethoxyphenylurea in succade.The sample is ultrasonic extracted with acetic acid/ammonium acetate buffer solution and purified by automatic solid-phase extraction.The extract is separated by SB-C1 8 column and detected by fluorescence detector.The value of correlation coefficient in the range of 0 to 10 mg/L is 0.9987.The limit of quantity （S/N=10）is less than 0.1 mg/kg.Using blank sample of succade as matrixes,the recovery is tested at 3 different concentration levels and the values of recovery are in the range of 81.7% to 92.4% with RSDs （n=6）in the range of 2.4% to 6.8%.

关 键 词：全自动固相萃取 高效液相色谱 蜜饯 对乙氧基苯脲 

分 类 号：TS202.3[轻工技术与工程—食品科学]