胃癌中幽门螺杆菌L型感染和淋巴管生成因子与淋巴结转移的关系  

Association of Helicobacter Pylori L-form Infection and Lymphangiogenesis Factors with Lymph Node Metastasis of Gastric Cancer

作　　者：蔡兆根[1,2] 于东红[1,2] 吴海波 冯振中[1,2] 赵艳[1,2] 

机构地区：[1]蚌埠医学院第一附属医院临床病理科 [2]蚌埠医学院病理学教研室,安徽省感染与免疫重点实验室,蚌埠233003 [3]安徽医科大学附属省立医院临床病理科,合肥230001

出　　处：《华中科技大学学报（医学版）》2014年第1期32-38,共7页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

基　　金：安徽省教育厅自然科学研究项目(No.KJ2011B097)

摘　　要：目的研究胃癌组织中血管内皮生长因子-C、-D、-A(VEGF-C、-D、-A)及成纤维细胞生长因子-2(FGF-2)的表达和幽门螺杆菌L型(helicobacter pylori L-form,Hp-L型)感染与淋巴结转移的关系。方法应用革兰染色和免疫组化SP法检测112例胃癌组织和30例对照组中的Hp-L型感染情况,同时免疫组化SP法检测上述组织中VEGF-C、-D、-A及FGF-2的表达情况及D2-40标记的微淋巴管密度值(LVD值),分析Hp-L型和淋巴管生成因子表达的关系及与LVD值的关系。结果胃癌组织中革兰染色Hp-L型检出率为65%,免疫组化Hp-L型抗原表达率为62%。胃癌组的Hp-L型阳性率、淋巴管生成因子阳性率及LVD值均高于对照组(均P<0.01);胃癌组中Hp-L阳性组的LVD值和VEGF-C、-D的表达阳性率均高于Hp-L阴性组(均P<0.05);VEGF-C、-D阳性组的LVD值高于阴性组(均P<0.05)。多因素分析发现,TNM分期和LVD值与胃癌淋巴结转移密切相关(均P<0.05)。结论 Hp-L型感染和淋巴管生成因子与胃癌的发生、发展相关,VEGF-C、-D与胃癌淋巴管生成关系密切,Hp-L型感染在肿瘤淋巴管生成和淋巴结转移方面具有一定作用,两方面因素共同促进胃癌的侵袭和转移。Objective To investigate the association of the expression of vascular endothelial growth factors-C,-D, A （VEGF-C,-D, A） and transforming growth factor-2 （FGF-2）, and the helicobacter pylori L-form （Hp-L） infection with lymph node metastasis in gastric cancer tissues. Methods Gram staining and immunohistochemistry were used to detect the Hp-L in- fection in 112 gastric carcinoma tissues and 30 normal gastric tissues. The expression of VEGF-C,-D,-A, and FGF-2, and lym- phatic vessel density（LVD）（defined as the expression of D2-40）were immunohistochemically detected. The relationships among the Hp-L infection, the expression of the lymphangiogenesis factors and the LVD were analyzed. Results Gram staining showed that the positive rate of Hp-L in gastric carcinoma was 65% ,which was similar to 62% of Hp-L infection on immuno- histochemical staining. The Hp-L infection rate,the expression of the lymphangiogenesis factors and the LVD were much higher in gastric carcinoma tissues than in normal gastric tissues（P〈0.01 for all）. In the gastric carcinoma tissues, the expression of lymphangiogenesis factors and the LVD were significantly higher in Hp-L positive group than in Hp-L negative group（P〈 0.05）. The LVD was related to the lymph node metastasis, and it was significantly increased in VEGF-C/-D-positive group when compared with VEGF C/-D-negative group（P〈0.05）. The positive rates of VEGF-C/-D were obviously higher in gastric carcinoma tissues with lymph node metastasis than in those without lymph node metastasis（P〈0.05）. The multivariate analysis findings revealed that the TNM stage and the LVD were closely correlated with lymph node metastasis of gastric carcino- ma. Conclusion Hp L infection and lymphangiogenesis factors are associated with the occurrence and progression of gastric carcinoma. The expression of VEGF C/-D is closely correlated with the lymphangiogenesis in gastric carcinoma. Hp-L infection may play a certain role in tumor lymphangiogenesis and lymph node me

关 键 词：胃癌 幽门螺杆菌L型 微淋巴管密度 淋巴管生成因子 

分 类 号：R735.2[医药卫生—肿瘤]