高效液相色谱柱后衍生法测定中药材中的黄曲霉毒素  

Determination of Aflatoxins in 4 Kinds of Traditional Chinese Medicines in Puer Downtown Areas by HPLC with Post-column Derivatization

作　　者：王娅玲[1] 李维峰[1] 刘宝 刘香 何维红 

机构地区：[1]云南热带作物职业学院热带作物生产与技术系,云南普洱665000 [2]普洱淞茂制药股份有限公司,云南普洱665000

出　　处：《云南化工》2014年第1期42-44,共3页Yunnan Chemical Technology

摘　　要：检测普洱城区僵蚕、陈皮、桃仁、胖大海4种中药材的黄曲霉毒素,了解其污染状况。样品经70%甲醇提取、免疫亲和柱净化后,用HPLC-柱后衍生-荧光检测器进行分析测定药材中的黄曲霉毒素(G2、G1、B2、B1)含量。检测的80个样品中,共有7个样品检出黄曲霉毒素,黄曲霉毒素B1的含量最高为1.89μg/kg,黄曲霉毒素总量最高为3.57μg/kg。尽管未超出国家药典规定范围,但是普洱城区4种中药材存在一定的黄曲霉毒素污染的风险。Objective： To determine aflatoxins in 4 kinds of Traditional Chinese Medicines including Bombyx batryticatus, dried tangerine peel, Persicae Semen, Sterculia lychnophora from Puer Downtown Areas. Method： After being extracted by 70% methanol and purified by immunoaffinity columns, 4 aflatoxins （ G2, G1, B2, B1 ） from 80 samples of 4 kinds of Traditional Chinese Medicines were analyzed by HPLC with post - column derivatization and fluorescence detector. Result： The aflatoxins were found in 5 samples, with positive rate of 8.75% in all. The highest content of aflatoxin B1 is 1.89μg/kg,and the highest content of aflatoxins is 3.57μg/kgo Conclusion ： The aflatoxins pollution exists among the 4 kinds of Traditional Chinese Medicines from Puer Downtown Areas, although it is lower than the national standard.

关 键 词：僵蚕 陈皮 桃仁 胖大海 黄曲霉毒素 高效液相色谱柱后衍生法 

分 类 号：R927.11[医药卫生—药学]