酿酒酵母工程菌产3-甲硫基丙醇的发酵条件优化  

Optimization of fermentation conditions for 3-methylthiopropanol production by recombinant Saccharomycescerevisiaestrain

作　　者：杨雪莲[1,2] 张凯丽[1,2] 李金玉[1,2] 张秋晨[1,2] 罗霞[1,2] 王成涛[1,2] 

机构地区：[1]北京工商大学北京市食品添加剂工程技术研究中心,北京100048 [2]北京工商大学食品添加剂与配料北京高校工程研究中心,北京100048

出　　处：《河北农业大学学报》2014年第1期94-101,共8页Journal of Hebei Agricultural University

基　　金：国家自然科学基金项目(31301410);北京市自然科学基金项目(5122008);大学生科研与创业行动计划(SJ201302059)

摘　　要：为提高天然香料3-甲硫基丙醇的得率,本研究采用单因素和正交设计实验对表达脱羧酶酿酒酵母工程菌的发酵条件进行优化。确定了工程菌的最佳发酵培养基为:蛋氨酸5g/L,微量元素2%(v/v),酵母提取物4g/L,蛋白胨12g/L,KH2PO410g/L,K2HPO42g/L,葡萄糖20g/L,G418 400μg/L。最适发酵条件为:接种量10%,pH为5,发酵温度30℃,转速200r/min。优化后3-甲硫基丙醇的浓度可达4.38g/L,较初始条件提高了3.5倍。In order to improve the yield of 3-methylthiopropanol,the fermentation conditions of recombinant Saccharomyces cerevisiae strain were optimized by single factor test and orthogonal design. The optimal fermentation medium was determined as follows： methionine 5 g/L , trace elements 2% （v/v）, yeast extract 4 g/L, peptone 12 g/L, KHzPO4 10 g/L, KzHPO4 2 g/L, glucose 20 g/L, G418 400μg/L. The optimal fermentation conditions were： inocula- tion volume 10%, 50 mL liquid culture in 250 mL flask, temperature 30℃, rotation speed 200 r/min and initial pH 5.0. Under this optimum condition, the concentration of 3-methylthiopro-panol of 4.38 g/L was achieved, which was 3.5 times higher than before.

关 键 词：3-甲硫基丙醇 酿酒酵母工程菌 正交实验 发酵优化 

分 类 号：TS2[轻工技术与工程—食品科学与工程]