经典霍奇金淋巴瘤MUM1和CD99及PRDM1表达与临床意义分析  

Expression and clinical significance of MUM1,CD99and PRDM1in classic Hodgkin lymphoma

作　　者：周新华[1,2] 黄学平[2] 钟琳[2] 赵彤[1,2] 

机构地区：[1]南方医科大学南方医院病理科,广东广州510515 [2]南方医科大学基础医学院病理学系,广东广州510515

出　　处：《中华肿瘤防治杂志》2014年第3期169-173,共5页Chinese Journal of Cancer Prevention and Treatment

基　　金：国家自然科学基金(81071941;81302051);广东省自然科学基金(S2013040016587)

摘　　要：目的:检测MUM1、CD99和PRDM1在经典霍奇金淋巴瘤(classic Hodgkin lymphoma,CHL)肿瘤细胞中的表达以及相互关系,分析其在H/RS细胞形成中的作用及临床意义。方法:采用免疫组化的方法检测62例CHL组织MUM1、CD99和PRDM1的表达,蛋白质印迹法检测CD99和PRDM1在6株B细胞来源和3株T细胞来源淋巴瘤细胞的表达;蛋白质印迹法检测CHL细胞株L428过表达CD99后以及重新抑制CD99后PRDM1表达。结果:62例CHL组织,61例(98.4%)MUM1呈强阳性高表达,1例(1.6%)CD99在H/RS细胞表达阳性,且为30%以下的H/RS细胞表达,PRDM1均呈阴性表达,阳性表达率差异有统计学意义,χ2=177.145,P<0.001;蛋白质印迹法结果显示,CD99和PRDM1在多发性浆细胞性骨髓瘤细胞株RPMI-8226中表达较高,在包括L428细胞在内的其他B细胞来源的淋巴瘤细胞株中表达较低;L428过表达CD99后PRDM1表达增加;重新抑制CD99表达后PRDM1表达消失。结论:H/RS细胞具有浆细胞分化潜能,CD99和PRDM1在H/RS细胞的形成中存在紧密的内在联系,上调L428中CD99的表达可能恢复PRDM1的失活,诱导H/RS细胞向成熟B细胞方向分化,结果对临床开展霍奇金淋巴瘤的诱导分化治疗具有指导意义。OBJECTIVE：To explore differences of MUM1,CD99 and PRDM1 expression in Classic Hodgkin lympho- ma and the interaction between them,and reveal the role of them in H/RS cell generation. METHODS： Sixty-two＇speci- mens of CHL cases were collected and analyzed by immunohistochemistry of MUM1, CD99 and PRDM1. CD99 and PRDM1 protein expression were further measured by Western blot and immunocytochemistry in six B-cell origin and three T-cell origin lymphoma cell lines. The expression of PRDM1 protein was analyzed by western blot in L428 cells with over- expression of CD99 and L428-CD99 cells with transfection of an siRNA targeted CD99. RESULTS： Positive for MUM1 was highly observed in 61 （98.4%） of 62 CHL cases（x2 =177. 145,P^0. 001）. In 1 of the 62 CHL cases （1.6%） examined, positivity for CD99 was detected in 30~ or fewer of the H/RS cells. PRDM1 expression was not found in H/RS cells of 62 CHL cases. CD99 and PRDM1 were highly expressed in multiple myeloma （MM） cell line RPMI-8226 ,and lowly or ab- sently expressed in L428 cell and other B-cell origin of lymphoma cell lines. We found that L428 cells with overexpression of CD99 showed a concomitant increase expression of PRDM1 protein by western blot analysis, when an siRNA targeted CD99 was transfected into L428-CD99 cells. This transfection in turn caused the decrease of PRDM1 protein level,as as- sessed by WB. CONCLUSIONS： H/RS cells have the potential of plasma-cell differentiation. CD99 and PRDM1 is tightlyrelated to generation and development of CHL,and CD99 is able to regulate the PRDM1 to induce H/RS cells differentia tion towards terminal B ceils. （）ur study may provide a new therapeutic approach for inducing CHL differentiation.

关 键 词：经典霍奇金淋巴瘤 H RS细胞 MUM1 CD99 PRDM1 

分 类 号：R733.1[医药卫生—肿瘤]