一种新的鼠大脑局部缺血模型  

A new focal cerebral ischemia model of rats

作　　者：王跃民[1] 谢安[1] 臧益民[1] 朱妙章[1] 

机构地区：[1]第四军医大学基础部生理学教研室,陕西西安710033

出　　处：《第四军医大学学报》2001年第3期284-286,共3页Journal of the Fourth Military Medical University

基　　金：国家自然科学基金资助项目 !(3 9970 2 0 4)

摘　　要：目的　制成鼠大脑局部缺血模型 .方法　采用左侧颈动脉离心端插管 ,灌注一定压力和流量的生理盐水 .结果　 1美蓝染色显示大脑左侧大部分区域被染蓝 ,除皮层着色较浅外 ,其余部位着色较深 . 2体感诱发电位在灌注后 30min时 ,其幅度超过灌注前 2 0 % .结论　染色说明大脑左侧为缺血区 ,体感诱发电位幅度的增高 ,表明大脑处于兴奋性损伤状态 .AIM To make a new focal cerebral ischemia model of rats. METHODS By perfusing normal saline from the catheter inserted in the left common carotid artery with certain pressure and flow. RESULTS Methylene blue staining showed that most areas in left cerebrum became dark blue except that cortex became slight blue. 30 min after infusion, the amplitude of somato sensory evoked potential (SEP) increases by more than 20%. CONCLUSION Staining indicates that left cerebrum becomes ischemia. The augment of SEP amplitude says that cerebrum is in excitotoxic injury state. It has been proved that this regional cerebral ischemia model is reliable and easy to carry out.

关 键 词：脑缺血 诱发电位 动物疾病模型 大鼠 

分 类 号：R743.31[医药卫生—神经病学与精神病学]