纤维连接蛋白对卵巢癌多细胞团簇形成的影响  

The Influence of Fibronectin on the Formation of Multi-cellular Spheroid of Ovarian Cancer

作　　者：谢晓砚[1,2,3] 刘珊玲[1,2,3] 王和[4,2,3] 林琳[1,2,3] 朱红梅[2,3] 张建军[2,3] 郑莹[2,3] 

机构地区：[1]四川大学华西第二医院西部妇幼医学研究院细胞与基因治疗实验室,成都610041 [2]四川大学华西第二医院妇产科教研室,成都610041 [3]四川大学出生缺陷与相关妇儿疾病教育部重点实验室,成都610041 [4]四川大学华西第二医院西部妇幼医学研究院遗传实验室,成都610041

出　　处：《四川大学学报（医学版）》2014年第2期240-244,共5页Journal of Sichuan University(Medical Sciences)

基　　金：国家自然科学基金(No.81074142;No.81270660);四川省科技厅科技支撑计划(No.2012SZ0136)资助

摘　　要：目的初步探索纤维连接蛋白对卵巢癌多细胞团簇形成的影响及可能参与的整合素受体亚型。方法以卵巢癌细胞系SKOV3为对象,在体外建立多细胞团簇模型,研究纤维连接蛋白对团簇形成的影响,在此基础上再用荧光定量PCR和免疫蛋白印迹从mRNA和蛋白水平上检测因纤维连接蛋白刺激而表达改变的整合素受体亚型。结果纤维连接蛋白能够促进大于250μm的多细胞团簇的形成;纤维连接蛋白刺激形成的多细胞团簇中整合素受体亚型ITGA5的表达受到了抑制。结论本研究证实了纤维连接蛋白能促进卵巢癌多细胞团簇的形成,并初步提示整合素受体亚型ITGA5很可能参与了这一影响过程。Objective To investigate the role of Fibronectin in the formation of multi-cellular spheroid of ovarian cancer and the integrin receptor involved in the process. Methods In vitro model of multi-cellular spheroid of SKOV3 was constructed by liguid overlay technique. The influence of fibronectin on the formation of the spheroid was observed. The gene expressions of potential integrin receptors were examined from the levels of mRNA and protein using real time reverse transcription PCR and Western-blot. Results Fibronctin stimulated the formation of multi-cellular spheroid of ovarian cancer larger than 250 ~m. fibronectin suppressed the expression of subtype of integrin receptor ITGA5. Conclusion Fibronectin can enhance the formation of multi-cellular spheroid of ovarian cancer. The subtype of integrin receptor ITGA5 is probably involved in the process.

关 键 词：卵巢癌 多细胞团簇 纤维连接蛋白 整合素受体 

分 类 号：R737.31[医药卫生—肿瘤]