D101大孔树脂吸附大豆乳清废液中异黄酮的实验研究  

Adsorbing the isofl avones from soybean whey waste water with the D101 macroporous resin

作　　者：孙婕[1] 苏晶莹[2] 尹国友[1] 王超[1] 罗丹凤[1] 吉晓辉[1] 

机构地区：[1]河南城建学院生命科学与工程学院,平顶山467044 [2]平顶山工业职业技术学院,平顶山467001

出　　处：《食品科技》2014年第3期202-206,共5页Food Science and Technology

基　　金：河南省科技计划重点科技攻关项目(112102210366)

摘　　要：以大豆乳清废液为原料,研究了用D101型大孔树脂分离和纯化大豆乳清中大豆异黄酮的工艺条件,分别进行了进行静态和动态吸附与解吸实验。实验结果表明,静态条件为:在pH3、2.0 g大孔树脂/50 mL样品液、吸附时间2.5 h条件下,达到最大吸附率66.4%;在乙醇浓度80%、料液比1:40、洗脱时间5 h,达到最大洗脱率77.7%,在最优工艺条件下回收率达35.6%,得率为0.0072 mg/mL,异黄酮纯度18.94%;动态条件为:最佳上样量为25 mL,流速1.0 mL/min,用90 mL 80%的乙醇基本洗脱完全,可实现较高浓度异黄酮的获得。The study with soybean whey waste as raw material, it studies systematically the craft of separation and purification process of soy isoflavones in soybean whey by using D101 macroporous resin. The results of static are as follows： at the condition of pH being 3, 2.0 g D101 macroporous resin/50 mL sample solution, adsorption time being 2.5 h, the maximum adsorption rate achieve as high as 66.4%. At the condition of ethanol concentration being 80%, liquid ratio being 1：40, elution time being 5 h, the elution rate is up to 77.7%, so the recycling rate will achieve 35.6% under the optimum conditions, with the yield being 0.0072 mg/mL, the purity of soybean isoflavones are 18.94%; the dynamic＇s result is： the optimal sample quantity is 25 mL, with 80% ethanol 90 mL to eluted completely, can acquire higher concentration of isoflavone.

关 键 词：大豆乳清 异黄酮 D101大孔树脂 动态和静态吸附与解吸 

分 类 号：R284.2[医药卫生—中药学]