蓝、紫粒小麦Wx基因分子检测及部分氨基酸含量分析  

Wx Gene Molecular Detection and Statistical Analysis on Part of the Amino Acid Content of Blue or Purple Grain Wheat

作　　者：任文斌[1] 谢三刚[1] 吴翠翠[1] 杨慧勇[2] 李素娟[3] 杨娜[1] 高明[1] 宋昱[1] 谢飒英[1] 

机构地区：[1]山西省农业科学院棉花研究所,山西运城044000 [2]山西省农业科学院高粱研究所,山西晋中030600 [3]山西省农业科学院现代农业研究中心,山西太原030031

出　　处：《华北农学报》2014年第1期103-107,共5页Acta Agriculturae Boreali-Sinica

基　　金：山西省科技攻关项目(20130311001-3);山西省农业科学院育种工程项目(11yzgc102)

摘　　要：利用3对引物对47份品系进行Wx基因分子检测,其中3份白粒小麦为野生型材料,22份蓝粒小麦和22份紫粒小麦为Wx基因全突变型材料,即全糯小麦。对其部分氨基酸含量进行多重比较,结果表明,总体上蓝粒糯小麦氨基酸含量高于紫粒糯小麦,紫粒糯小麦高于白粒小麦;3种类型小麦精氨酸、半胱氨酸、赖氨酸、蛋氨酸、苏氨酸、缬氨酸含量差异极显著;异亮氨酸、色氨酸含量差异达显著水平。Three primer pairs of Wx gene were used in molecular detection from forty-seven wheat strains. Three white wheats were wild type material,twenty-two Blue grain and twenty-two purple grain of wheats were Wx gene mutation type materials,named the full waxy wheats. Multiple comparative analysis on part of the amino acid con-tent. The results showed that amino acid content of the blue waxy wheats was higher than the purple waxy wheats, the purple waxy wheats was higher than white wheats;Arg, Cys, Lys, Met, Thr, Val content of the three types of wheat were extremely significant different,Ile,Trp content were significantly different.

关 键 词：蓝粒小麦 紫粒小麦 WX基因 氨基酸 

分 类 号：S512.1[农业科学—作物学]