纤维素酶解滁菊提取多糖工艺及滁菊多糖抗氧化性研究  

Extracting the polysaccharides from Dendranthema morifolium by cellulase degradation and determining the antioxidant activities of polysaccharides

作　　者：熊磊[1] 年四辉[2] 朱玉[3] 

机构地区：[1]皖南医学院生化教研室,安徽芜湖241002 [2]皖南医学院药学院,安徽芜湖241002 [3]皖南医学院公共卫生学院,安徽芜湖241002

出　　处：《皖南医学院学报》2014年第1期23-26,共4页Journal of Wannan Medical College

基　　金：院中青年科研基金项目(WK201106)

摘　　要：目的:从滁菊醇提药渣中用纤维素酶法提取多糖,研究滁菊多糖抗氧化性。方法:分别考察酶解pH值、酶解温度、酶用量、酶解时间4个因素对滁菊多糖得率的影响。结果:通过正交试验得出滁菊多糖的最佳提取工艺为:酶解pH 5.5、温度60℃、酶用量20 U/g、酶解时间100 min,多糖得率为13.89%。结论:对DPPH自由基和超氧阴离子自由基的清除率表明滁菊多糖具有较好的抗氧化活性。Objective：To tentatively verify the process for further extracting the polysaccharides from flower residue of Dendranthema morifolium Tzvel. （DMP,treated previously with ethanol） with cellulase degradation technique and assay the antioxidant activities of DMP.Methods：The influential factors affecting the recovery rate in extracting polysaccharides were determined regarding the pH value,temperature at enzymolysis,enzyme dosage and extraction time.Results：By orthogonal test analysis,the results showed that the optimal conditions were involved in pH value by 5.5,temperature at 60 ℃,enzyme dosage in 20 U/g and extraction time for 100 min in a typical full extracting process,which led to recovery rate of 13.89%.Conclusion：The experimental findings show that DMP may facilitate stronger antioxidant activities based on measurement of the scavenging effect on DPPH and superoxide anion free radical.

关 键 词：滁菊药渣 多糖 纤维素酶 提取 抗氧化性 

分 类 号：R284.2[医药卫生—中药学]