酶联免疫法测定鸡肉中尼卡巴嗪残留  

Enzyme- linked Immunosorbent Assay for Detection of Nicarbazin Residue in Chicken Muscle

作　　者：王鹤佳[1] 刘智宏[1] 汪霞[1] 黄耀凌[1] 孙雷[1] 

机构地区：[1]中国兽医药品监察所,北京100081

出　　处：《中国兽药杂志》2014年第3期59-61,共3页Chinese Journal of Veterinary Drug

基　　金：国家科技支撑计划(2011BAK10B01-11)

摘　　要：为了建立鸡肉中尼卡巴嗪残留标志物4，4’-二硝基均二苯脲（DNC）残留酶联免疫（ELISA）检测方法。将N-琥珀酰-L-丙氨酰-L-丙氨酰-L-丙氨酸4-硝基苯胺（SAN）与载体蛋白偶联作为抗原免疫动物，获得抗DNC抗体，在此基础上建立了鸡肉中尼卡巴嗪残留的检测方法。人工抗原中SAN与载体蛋白的结合比约为12：1，血清效价1：2000倍，鸡肉中检测限为9．2μg／kg，添加回收率在49．4％～118％之间，批内、批间变异系数均小于20％。该方法在灵敏度、精密度和准确度方面均能满足兽药残留筛选检测要求。ELISA for detection of 4, 4 ＇ - dinitrocarbanilide （ DNC ） , marker residue of nicarbazin, in chicken muscle was developed. Anti-DNC antibody was gotten by immunizing animals with SAN-protein combination. The conjugate rate of SAN and protein was 12 ： 1 in artificial antigen. Antiserum valence was more than 1 ： 2000. The limit of detection was 9.2 μg/kg in chicken muscle. The recovery rate range was from 49.4% to 118%. The sensibility, precision and accuracy of the method could satisfy the request of screening veterinary drug residue

关 键 词：尼卡巴嗪 4 4’-二硝基均二苯脲 残留 鸡肉 酶联免疫法 

分 类 号：S859.84[农业科学—临床兽医学]