沙冬青抗冻蛋白热滞活性的DSC研究  

DSC Study on the Thermal Hysteresis Activity of Plant Antifreeze Proteins

作　　者：周晓蕾[1] 陈滔滔[1] 王保怀[1] 李芝芬[1] 高素琴[1] 费云标[2] 魏令波[2] 

机构地区：[1]北京大学物理化学研究所,北京100871 [2]中国科学院发育研究所,北京100080

出　　处：《物理化学学报》2001年第1期66-69,共4页Acta Physico-Chimica Sinica

基　　金：国家自然科学基金

摘　　要：Differential scanning calorimetry（ DSC） was used to measure the thermal hysteresis activity(THA) of plant antifreeze proteins(AFPs). The results reveal that DSC is a good method to screen and study AFPs. In the sixteen components extracted from Ammopipanthus mongolicus leaves, one(P3S1) was found to have apparent thermal hysteresis activity by DSC. As the amount of ice nuclei in the sample decreased, the THA of P3S1 increased from 0.01℃ to 0.65℃ . It is notable that the two- peak thermal hysteresis effect was observed. Two endothermic peaks appeared in the melting process of P3S1, while the freezing peak also consisted of two peaks. The peaks appeared antecedently showed larger thermal effect. This phenomenon shows P3S1 has two different kinds of interaction with water and ice crystal. It is probably an important property of a class of AFPs.Differential scanning calorimetry (DSC) was used to measure the thermal hysteresis activity(THA) of plant antifreeze proteins(AFPs). The results reveal that DSC is a good method to screen and study AFPs, In the sixteen components extracted from Ammopipanthus mongolicus leaves, one(P3S1) was found to have apparent thermal hysteresis activity by DSC. As the amount of ice nuclei in the sample decreased, the THA of P3S1 increased from 0.01 degreesC to 0.65 degreesC, It is notable that the two-peak thermal hysteresis effect was observed. Two endothermic peaks appeared in the melting process of P3S1, while the freezing peak also consisted of two peaks. The peaks appeared antecedently showed larger thermal effect. This phenomenon shows P3S1 has two different kinds of interaction with water and ice crystal. It is probably an important property of a class of AFPs.

关 键 词：抗冻蛋白 热滞活性 沙冬青 热滞机理 抗冻性 差示扫描量热法 

分 类 号：O629[理学—有机化学]