血清GSTP1基因甲基化定量分析在肝细胞癌的早期诊断价值  

Value of serumGSTP1 gene quantitative methylation analysis for early diagnosis of hepatocellular carcinoma

作　　者：冉贵萍[1,2] 杨国珍[2] 方文[2] 袁勇[1] 张瑞霞[1] 

机构地区：[1]上海交通大学医学院附属第九人民医院奉城分院,上海201411 [2]贵阳医学院附院生化科,贵州贵阳550004

出　　处：《国际检验医学杂志》2014年第5期540-542,共3页International Journal of Laboratory Medicine

基　　金：国家级教学团队资助项目(教高函[2009]18号)

摘　　要：目的建立实时荧光定量甲基化方法研究肝细胞癌(HCC)患者外周血血清中游离谷胱甘肽-硫-转移酶P1基因(GSTP1)启动子区域甲基化状态,探讨其是否可作为HCC早期诊断的指标。方法收集95例血清标本,包括40例HCC、30例肝硬化和25例健康体检者,采用实时荧光定量甲基化特异性聚合酶链反应(PCR)技术对血清中GSTP1基因的甲基化水平进行定量分析,应用接受者操作特性(ROC)曲线评估其诊断价值。结果肝癌患者血清中GSTP1基因甲基化定量水平明显高于健康对照组,差异有统计学意义(P<0.05)。ROC曲线分析表明通过GSTP1基因甲基化定量分析能够高效区分肝癌和肝硬化及健康者(AUC=0.864 1),以甲基化率2%作为诊断HCC的临界值,其诊断特异度为87.5%,敏感度达69.6%;联合检测血清GSTP1基因甲基化和血清AFP可将HCC检出率提高至75%。结论实时荧光定量甲基化方法可精确定量血清中GSTP1基因甲基化水平,在HCC的早期诊断中有一定的应用价值。Objective To establish a real-time fluorescence quantitative methylation assay to investigate the methylation status of GSH-sulphur-transferase P1（GSTP1） gene promoter region in hepatocellular carcinoma（HCC） and to investigate whether which can be used as the early diagnostic indicator of HCC .Methods Ninety-five serum samples were collected from 40 patients with HCC ,30 patients with liver cirrhosis and 25 individuals with healthy physical examination as controls .The methylation level of GSTP1 gene in these serum samples were quantitatively determined by using the real-time fluorescence quantitative methylated spe-cific PCR technique .The receiver-operation characteristic（ROC） curves were adopted to evaluate its diagnostic value for HCC .Re-sults The methylation quantitative level of GSTP1 gene in HCC serum was significantly higher than that in the healthy controls （P＆lt;0 .05） .The ROC curve analysis demonstrated that the methylation quantitative analysis of GSTP1 gene could efficiently distin-guish HCC and cirrhosis from healthy controls （AUC=0 .8641） .With the methylation rate of 2% as the critical value for diagno-sing HCC ,its diagnostic specificity was 87 .5% ,the sensitivity was 69 .6% ;the combination detection of serum GSTP1 gene methy-lation and serum AFP could increase the detection rate of HCC to 75% .Conclusion The real-time fluorescence quantitative methyl-ation assay can accurately quantify the methylation level of serum GSTP1 gene ,which has certain application value for the early di-agnosis of HCC .

关 键 词：癌  肝细胞 谷胱甘肽转移酶 DNA甲基化 

分 类 号：R735.7[医药卫生—肿瘤]