应用免疫组织化学法检测14-3-3ε和Peroxiredoxin 1在骨巨细胞瘤中的表达  

Application of immunohistochemical in detecting of expression and clinical significance of 14-3-3ε and Peroxiredoxin 1 in giant cell tumor of bone

作　　者：李颖智[1] 金海鸿[1] 胡惠静[1] 秦悦洋[1] 李成镇[1] 毛铁[1] 李敏[1] 季宏波[1] 王友成[1] 

机构地区：[1]黑龙江省医院骨外科,哈尔滨150036

出　　处：《国际免疫学杂志》2014年第2期165-168,共4页International Journal of Immunology

基　　金：基金项目：黑龙江省科技攻关项目（GZllC007）

摘　　要：目的 探讨14-3-3ε和Peroxiredoxin 1在良性与侵袭性骨巨细胞瘤组织中的表达差异及临床意义.方法 采用免疫组织化学方法检测分析14-3-3ε和Peroxiredoxin 1在13例良性与1 1例侵袭性骨巨细胞瘤组织表达差异.结果 14-3-3ε在单核基质细胞、多核巨细胞胞浆内均有表达,在良性与侵袭性骨巨细胞瘤组织单核基质细胞的强阳性表达率分别为23.08％、63.63％,差异无统计学意义（P =0.0953）,但侵袭性骨巨细胞瘤中存在14-3-3￡在多核巨细胞胞浆内高表达现象,个别病例多核巨细胞细胞核染色阳性而胞浆染色弱.Peroxiredoxin 1在单核基质细胞、多核巨细胞均有表达,多为细胞浆染色,在良性与侵袭性骨巨细胞瘤组织单核基质细胞的强阳性表达率分别为61.54％ 、18.18％,差异有统计学意义（P =0.0472）.结论 14-3-3ε和Peroxiredoxin 1可能与骨巨细胞瘤发生、发展、侵袭性相关,联合检测14-3-3ε和Peroxiredoxin 1可能有助骨巨细胞瘤治疗、预后判断.Objective To study the expression and clinical significance of 14-3-3ε and Peroxiredoxin 1 in giant cell tumor of bone.Methods The expression of 14-3-3ε and Peroxiredoxin 1 were examined in 13 cases of benign giant cell tumor of bone and 11 cases of aggressive giant cell tumor of bone by immunohisto chemical.Results In benign and aggressive giant cell tumor of bone,the intense positive rate for 14-3-3ε were 23.08% and 63.63% （P =0.953）.The intense positive rate for Peroxiredoxin 1 were 61.54% and 18.18%,respectively.There was significant difference in the rates of expression （P =0.0472）.Conclusion The expression of 14-3-3ε and Peroxiredoxin 1 were positively related to the invasiveness and prognosis of giant cell tumor of bone.The combined detection of 14-3-3εand Peroxiredoxin 1 may be valuable for predicting the prognosis and guiding the therapy for the patients of giant cell tumor of bone.

关 键 词：免疫组化 骨巨细胞瘤 14-3-3ε PEROXIREDOXIN 1 14-3-3 ε PEROXIREDOXIN 1 

分 类 号：R738.1[医药卫生—肿瘤]