Short-term environmental enrichment exposure induces proliferation and maturation of doublecortin-positive cells in the prefrontal cortex  

作　　者：Chunling Fan Mengqi Zhang Lei Shang Ngobe Akume Cynthia Zhi Li Zhenyu Yang Dan Chen Jufang Huang Kun Xiong 

机构地区：[1]Department of Anatomy and Neurobiology, Central South University School of Basic Medical Sciences [2]Grade 2006, Eight-year Medicine Doctor Program, Central South University Xiangya School of Medicine [3]Grade 2011, Six-year Medicine Program of International Student, Central South University Xiangya School of Medicine [4]Grade 2008, Eight-year Medicine Doctor Program, Central South University Xiangya School of Medicine

出　　处：《Neural Regeneration Research》2014年第3期318-328,共11页中国神经再生研究（英文版）

基　　金：the National Natural Science Foundation of China,No.30900773;the Natural Science Foundation of Hunan Province in China,No.11JJ2020;Young Teachers Training Program of University of Hunan Province

摘　　要：Previous studies have demonstrated that doublecortin-positive immature neurons exist pre- dominantly in the superficial layer of the cerebral cortex of adult mammals such as guinea pigs, and these neurons exhibit very weak properties of self-proliferation during adulthood under physiological conditions. To verify whether environmental enrichment has an impact on the proliferation and maturation of these immature neurons in the prefrontal cortex of adult guinea pigs, healthy adult guinea pigs were subjected to short-term environmental enrichment. Animals were allowed to play with various cognitive and physical stimulating objects over a period of 2 weeks, twice per day, for 60 minutes each. Immunofluorescence staining results indicated that the number of doublecortin-positive cells in layer II of the prefrontal cortex was significantly increased after short-term environmental enrichment exposure. In addition, these doublecortin-positive cells co-expressed 5-bromo-2-deoxyuridine （a marker of cell prolifera- tion）, c-Fos （a marker of cell viability） and NeuN （a marker of mature neurons）. Experimental findings showed that short-term environmental enrichment can induce proliferation, activation and maturation of doublecortin-positive cells in layer II of the prefrontal cortex of adult guinea pigs.Previous studies have demonstrated that doublecortin-positive immature neurons exist pre- dominantly in the superficial layer of the cerebral cortex of adult mammals such as guinea pigs, and these neurons exhibit very weak properties of self-proliferation during adulthood under physiological conditions. To verify whether environmental enrichment has an impact on the proliferation and maturation of these immature neurons in the prefrontal cortex of adult guinea pigs, healthy adult guinea pigs were subjected to short-term environmental enrichment. Animals were allowed to play with various cognitive and physical stimulating objects over a period of 2 weeks, twice per day, for 60 minutes each. Immunofluorescence staining results indicated that the number of doublecortin-positive cells in layer II of the prefrontal cortex was significantly increased after short-term environmental enrichment exposure. In addition, these doublecortin-positive cells co-expressed 5-bromo-2-deoxyuridine （a marker of cell prolifera- tion）, c-Fos （a marker of cell viability） and NeuN （a marker of mature neurons）. Experimental findings showed that short-term environmental enrichment can induce proliferation, activation and maturation of doublecortin-positive cells in layer II of the prefrontal cortex of adult guinea pigs.

关 键 词：nerve regeneration NEUROGENESIS prefrontal cortex NEOCORTEX guinea pig doublecortinprotein cell proliferation neurons 5-bromodeoxyuridine NSFC grant neural regeneration 

分 类 号：R363[医药卫生—病理学]