结直肠癌中lincRNAs表达特征的初步研究  

Aberrant expression of long intergenic non-coding RNAs in human colorectal cancer

作　　者：古志强[1] 唐健[2] 黄凤婷[1] 庄燕妍[1] 彭娟菲 姚和瑞[3] 张世能[1] 

机构地区：[1]中山大学孙逸仙纪念医院消化内科,广东广州510120 [2]中山大学附属第六医院消化内科,广东广州510655 [3]中山大学孙逸仙纪念医院肿瘤科,广东广州510120

出　　处：《肿瘤》2014年第3期245-252,共8页Tumor

基　　金：广东省自然科学基金(编号:8151008901000139);广州市科技计划项目(编号:2009Y-C011-1)

摘　　要：目的 :探讨结直肠癌中长链基因间非编码RNAs(long intergenic non-coding RNAs,lincRNAs)的表达特征。方法 :采用基因芯片技术检测4例结直肠癌及癌旁正常黏膜组织中长链非编码RNAs(long non-coding RNAs,lncRNAs)与mRNAs的表达情况,筛选出差异表达的lincRNAs与mRNAs并用MeV 4.6软件行聚类分析;进一步采用上海伯豪生物技术有限公司的生物芯片分析系统(SBC Analysis System,SAS)分析其相关信号转导通路;采用实时荧光定量-PCR方法验证linc-UCC、linc-DCC与MIR143HG在癌组织及癌旁组织中的表达情况,并分析与结直肠癌临床特征之间的相关性。结果 :采用基因芯片共检测到42 545个RNA,其中124个lincRNAs和1 583个mRNAs的表达有明显差异。生物信息学分析结果显示,有29条信号转导通路可能与这些lincRNAs有关。linc-UCC在较多结直肠癌组织中表达增多,其高表达与肿瘤淋巴结转移密切相关;而linc-DCC与MIR143HG在多数结直肠癌组织中表达减少,二者呈正相关性。结论 :通过基因芯片技术筛选获得与结直肠癌相关的lincRNAs,以期为结直肠癌的临床诊断及治疗提供一些新的靶点。Objective： To investigate the expression profile of long intergenic non-coding RNAs （lincRNAs） in human colorectal cancer （CRC）. Methods： The expressions of long non-coding RNAs （IncRNAs） and mRNAs in four CRC tissues and para-cancerous normal colorectal mucosa tissues were detected by microarray analysis. Clustering heatmaps were made to profile the lincRNAs and mRNAs expressions by MeV 4.6 software. The signal pathways were analyzed by SBC Analysis System （SAS）. The linc-UCC, linc- DCC and MIR143HG expression levels were detected by real-time fluorogenic quantitative-PCR （RFQ-PCR）. Results： One hundred twenty-four lincRNAs and 1 583 mRNAs out of 42 545 RNAs detected by microarray assay were identified as differentially expressed between CRC tissues and para-cancerous normal colorectal mucosa tissues. Pathway analysis indicated that 29 potential pathways corresponded to these aberrant lincRNAs, linc-UCC was frequently up-regulated in CRC tissues and its high expression had a significant correlation with lymph node metastasis, linc-DCC and MIR143HG were down-regulated in CRC tissues and the positive correlation was shown between them. Conclusion： These results suggest that the differences in lincRNAs expression profiles may provide a new target for diagnosis and treatment of CRC.

关 键 词：结直肠肿瘤 长链非编码RNA 芯片分析技术 系统生物学 

分 类 号：R735.34[医药卫生—肿瘤]